Determination of cysteine in a pharmaceutical formulation by flow injection analysis with a chemiluminescence detector

被引：96

作者：

Lau, CW ^{[1
]}

Qin, XJ ^{[1
]}

Liang, HY ^{[1
]}

Lu, HZ ^{[1
]}

机构：

[1] Fudan Univ, Sch Pharm, Shanghai 200032, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2004年 / 514卷 / 01期

关键词：

cysteine; chemiluminescence; flow injection; pharmaceutical formulation;

D O I：

10.1016/j.aca.2004.01.007

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple and convenient flow injection-chemiluminescence (FI-CL) method for the determination of cysteine is reported, based on a fast and strong CL in a basic luminol-cysteine-NaIO4 solution. The linear range was 1.0 x 10(-8) to 1.0 X 10(-6) M with a detection limit (3s) of 5 x 10(-9) M, which was 100 times more sensitive than previously reported CL methods. Singlet oxygen, hydroxyl radical and hydrogen peroxide were suggested to be produced in this reaction and were responsible for the CL of cysteine. This simple method has been successfully applied for the determination of cysteine in a pharmaceutical formulation. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：45 / 49

页数：5

共 26 条

[1] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF URINARY CYSTEINE AND CYSTINE
BIRWE, H
HESSE, A
[J]. CLINICA CHIMICA ACTA, 1991, 199 (01) : 33 - 42
[2] Use of pH gradients in continuous-flow systems and multivariate regression techniques applied to the determination of methionine and cysteine in pharmaceuticals
Blasco, F
MedinaHernandez, MJ
Sagrado, S
[J]. ANALYTICA CHIMICA ACTA, 1997, 348 (1-3) : 151 - 159
[3] Daskalakis I, 1996, BIOMED CHROMATOGR, V10, P205, DOI 10.1002/(SICI)1099-0801(199609)10:5<205::AID-BMC589>3.3.CO
[4] 2-G
[5] Investigation on the chemiluminescence reaction of luminol-H2O2-S2-/R-SH system
Du, JX
Li, YH
Lu, JR
[J]. ANALYTICA CHIMICA ACTA, 2001, 448 (1-2) : 79 - 83
[6] EWING D, 1991, RADIAT RES, V28, P29
[7] Determination of reduced, protein-unbound, and total concentrations of N-acetyl-L-cysteine and L-cysteine in rat plasma by postcolumn ligand substitution high-performance liquid chromatography
Harada, D
Naito, S
Kawauchi, Y
Ishikawa, K
Koshitani, O
Hiraoka, I
Otagiri, M
[J]. ANALYTICAL BIOCHEMISTRY, 2001, 290 (02) : 251 - 259
[8] DETERMINATION OF CYSTEINE PLUS HALF-CYSTINE IN PROTEIN AND PEPTIDE HYDROLYSATES - USE OF DITHIODIGLYCOLIC ACID AND PHENYLISOTHIOCYANATE DERIVATIZATION
HOOGERHEIDE, JG
CAMPBELL, CM
[J]. ANALYTICAL BIOCHEMISTRY, 1992, 201 (01) : 146 - 151
[9] Determination of cysteine by capillary zone electrophoresis with end-column amperometric detection at a gold/mercury amalgam microelectrode without deoxygenation
Jin, WR
Wang, Y
[J]. JOURNAL OF CHROMATOGRAPHY A, 1997, 769 (02) : 307 - 314
[10] Resolution of amino thiols in time-resolved luminol chemiluminescence catalyzed by peroxidases
Kamidate, T
Tani, T
Watanabe, H
[J]. ANALYTICAL SCIENCES, 1998, 14 (04) : 725 - 729

← 1 2 3 →