LOXL1-AS1 contributes to the proliferation and migration of laryngocarcinoma cells through miR-589-5p/TRAF6 axis

被引：23
作者：
He, Guijun [1 ]
Yao, Wenfeng [2 ]
Li, Liang [1 ]
Wu, Yang [1 ]
Feng, Guojian [1 ]
Chen, Li [3 ]
机构：
[1] Lianyungang Second Peoples Hosp, Dept Otolaryngol & Head & Neck Surg, Lianyungang 222023, Jiangsu, Peoples R China
[2] First Peoples Hosp Xinxiang City, Dept Otolaryngol, Xinxiang 453000, Henan, Peoples R China
[3] Zaozhuang Municipal Hosp, Dept Otorhinolaryngol, 41 Longtou Middle Rd, Zaozhuang 277100, Shandong, Peoples R China
来源：
CANCER CELL INTERNATIONAL | 2020年 / 20卷 / 01期
关键词：
Laryngocarcinoma; LOXL1-AS1; miR-589-5p; TRAF6; LONG NONCODING RNAS; CANCER; PROGRESSION; INVASION; DRIVES;
D O I：
10.1186/s12935-020-01565-5
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Background LOXL1-AS1 is a long non-coding RNA (lncRNA) that plays crucial roles in various cancers. However, the functional role of LOXL1-AS1 in laryngocarcinoma remains unclear. Thus we planned to probe into the function and underlying mechanism of LOXL1-AS1 in laryngocarcinoma. Methods Gene expression was evaluated in laryngocarcinoma cells using RT-qPCR. The ability of cell proliferation and migration was assessed by CCK8, colony formation, wound healing and transwell assays. The interaction among LOXL1-AS1, miR-589-5p and TRAF6 was detected by Ago2-RIP, RNA pull down and luciferase reporter assays. Results LOXL1-AS1 was overexpressed in laryngocarcinoma cells. Silencing of LOXL1-AS1 suppressed cell proliferation, migration and EMT in laryngocarcinoma. Moreover, miR-589-5p, the downstream of LOXL1-AS1, directly targeted TRAF6 in laryngocarcinoma. Importantly, LOXL1-AS1 augmented TRAF6 expression in laryngocarcinoma cells by sequestering miR-589-5p. Besides, miR-589-5p worked as a tumor-inhibitor while TRAF6 functioned as a tumor-facilitator in laryngocarcinoma. Of note, rescue experiments both in vitro and in vivo validated that LOXL1-AS1 aggravated the malignancy in laryngocarcinoma by targeting miR-589-5p/TRAF6 pathway. Conclusions LOXL1-AS1 promotes the proliferation and migration of laryngocarcinoma cells through absorbing miR-589-5p to upregulate TRAF6 expression.
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页数：11
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