Genome sequence of Methylocystis hirsuta CSC1, a polyhydroxyalkanoate producing methanotroph

被引：13

作者：

Bordel, Sergio ^{[1
,2
]}

Rodriguez, Elisa ^{[1
,2
]}

Munoz, Raul ^{[1
,2
]}

机构：

[1] Univ Valladolid, Escuela Ingn Ind, Dept Ingn Quim & Tecnol Medio Ambiente, Valladolid, Spain

[2] Univ Valladolid, Inst Sustainable Proc, Valladolid, Spain

来源：

MICROBIOLOGYOPEN | 2019年 / 8卷 / 06期

基金：

欧盟地平线“2020”;

关键词：

genome; methane monooxygenases; methanotrophs; methylocystis; polyhydroxyalkanoates; METHANE ABATEMENT; ANNOTATION;

D O I：

10.1002/mbo3.771

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Polyhydroxyalkanoates (PHAs) are biodegradable plastics that can be produced by some methanotrophic organisms such as those of the genus Methylocystis. This allows the conversion of a detrimental greenhouse gas into an environmentally friendly high added-value bioproduct. This study presents the genome sequence of Methylocystis hirsuta CSC1 (a high yield PHB producer). The genome comprises 4,213,043 bp in 4 contigs, with the largest contig being 3,776,027 bp long. Two of the other contigs are likely to correspond to large size plasmids. A total of 4,664 coding sequences were annotated, revealing a PHA production cluster, two distinct particulate methane monooxygenases with active catalytic sites, as well as a nitrogen fixation operon and a partial denitrification pathway.

引用

页数：5

共 20 条

[1] Two isozymes of particulate methane monooxygenase with different methane oxidation kinetics are found in Methylocystis sp strain SCZ [J].

Baani, Mohamed ;

Liesack, Werner .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (29) :10203-10208

[2] antiSMASH 4.0-improvements in chemistry prediction and gene cluster boundary identification [J].

Blin, Kai ;

Wolf, Thomas ;

Chevrette, Marc G. ;

Lu, Xiaowen ;

Schwalen, Christopher J. ;

Kautsar, Satria A. ;

Duran, Hernando G. Suarez ;

Santos, Emmanuel L. C. de los ;

Kim, Hyun Uk ;

Nave, Mariana ;

Dickschat, Jeroen S. ;

Mitchell, Douglas A. ;

Shelest, Ekaterina ;

Breitling, Rainer ;

Takano, Eriko ;

Lee, Sang Yup ;

Weber, Tilmann ;

Medema, Marnix H. .

NUCLEIC ACIDS RESEARCH, 2017, 45 (W1) :W36-W41

[3]

Chin CS, 2013, NAT METHODS, V10, P563, DOI [10.1038/NMETH.2474, 10.1038/nmeth.2474]

[4] Genome Analysis Coupled with Physiological Studies Reveals a Diverse Nitrogen Metabolism in Methylocystis sp Strain SC2 [J].

Dam, Bomba ;

Dam, Somasri ;

Blom, Jochen ;

Liesack, Werner .

PLOS ONE, 2013, 8 (10)

[5] Complete Genome Sequence of Methylocystis sp Strain SC2, an Aerobic Methanotroph with High-Affinity Methane Oxidation Potential [J].

Dam, Bomba ;

Dam, Somasri ;

Kube, Michael ;

Reinhardt, Richard ;

Liesack, Werner .

JOURNAL OF BACTERIOLOGY, 2012, 194 (21) :6008-6009

[6]

Desai M., 2017, FED REGISTER, V82, P10767

[7] MUSCLE: multiple sequence alignment with high accuracy and high throughput [J].

Edgar, RC .

NUCLEIC ACIDS RESEARCH, 2004, 32 (05) :1792-1797

[8] Simultaneous methane abatement and PHB production by Methylocystis hirsuta in a novel gas-recycling bubble column bioreactor [J].

Garcia-Perez, Teresa ;

Lopez, Juan C. ;

Passos, Fabiana ;

Lebrero, Raquel ;

Revah, Sergio ;

Munoz, Raul .

CHEMICAL ENGINEERING JOURNAL, 2018, 334 :691-697

[9] Methanotrophic bacteria [J].

Hanson, RS ;

Hanson, TE .

MICROBIOLOGICAL REVIEWS, 1996, 60 (02) :439-+

[10]

Lebrero R, 2016, CHEMOSPHERE, V144, P97, DOI [10.1016/J.chemosphere.2015.08.017, 10.1016/j.chemosphere.2015.08.017]

← 1 2 →