Characterization of three Chlorella sorokiniana strains in anaerobic digested effluent from cattle manure

被引:105
作者
Kobayashi, Naoko [1 ]
Noel, Eric A. [2 ]
Barnes, Austin [3 ]
Watson, Andrea [3 ]
Rosenberg, Julian N. [4 ]
Erickson, Galen [3 ]
Oyler, George A. [1 ,4 ]
机构
[1] Univ Nebraska, Dept Biochem, Lincoln, NE 68588 USA
[2] Univ Nebraska, Sch Biol Sci, Lincoln, NE 68588 USA
[3] Univ Nebraska, Dept Anim Sci, Lincoln, NE 68588 USA
[4] Johns Hopkins Univ, Dept Chem & Biomol Engn, Baltimore, MD 21218 USA
关键词
Chlorella sorokiniana; Anaerobic digester effluent; Nutrient removal; Biomass; Starch; FRESH-WATER ALGAE; ELEMENTAL COMPOSITION; SWINE MANURE; WASTE-WATER; MICROALGAE; BIOMASS; RECOVERY; GROWTH; TRIACYLGLYCEROLS; REINHARDTII;
D O I
10.1016/j.biortech.2013.10.032
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
Chlorella sorokiniana CS-01, UTEX 1230 and UTEX 2714 were maintained in 10% anaerobic digester effluent (ADE) from cattle manure digestion and compared with algal cultivation in Bold's Basal Medium (BBM). Biomass of CS-01 and UTEX 1230 in ADE produced similar or greater than 280 mg/L after 21 days in BBM, however, UTEX 2714 growth in ADE was suppressed by more than 50% demonstrating a significant species bias to synthetic compared to organic waste-based media. The highest accumulation of protein and starch was exhibited in UTEX 1230 in ADE yielding 34% and 23% ash free dry weight (AFDW), respectively, though fatty acid methyl ester total lipid measured less than 12% AFDW. Results suggest that biomass from UTEX 1230 in ADE may serve as a candidate alga and growth system combination sustainable for animal feed production considering high yields of protein, starch and low lipid accumulation. (C) 2013 The Authors. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码:377 / 386
页数:10
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