Inhibitive effects of 15-deoxy-Δ12,14-prostaglandin J2 on hepatoma-cell proliferation through reactive oxygen species-mediated apoptosis

被引：8

作者：

Chen, Kan ^{[1
]}

Dai, Weiqi ^{[1
]}

Wang, Fan ^{[1
]}

Xia, Yujing ^{[1
]}

Li, Jingjing ^{[1
]}

Li, Sainan ^{[1
]}

Liu, Tong ^{[1
]}

Zhang, Rong ^{[2
]}

Wang, Jianrong ^{[2
]}

Lu, Wenxia ^{[2
]}

Zhou, Yuqing ^{[3
]}

Yin, Qin ^{[3
]}

Zheng, Yuanyuan ^{[1
]}

Abudumijiti, Huerxidan ^{[1
]}

Chen, Rongxia ^{[1
]}

Lu, Jie ^{[1
]}

Zhou, Yingqun ^{[1
]}

Guo, Chuanyong ^{[1
]}

机构：

[1] Tongji Univ, Sch Med, Shanghai Peoples Hosp 10, Dept Gastroenterol, Shanghai 200072, Peoples R China

[2] Nanjing Med Univ, Clin Med Coll 1, Tongji Univ, Dept Gastroenterol,Shanghai Peoples Hosp 10,Sch M, Nanjing, Jiangsu, Peoples R China

[3] Soochow Univ, Affiliated Hosp 1, Tongji Univ, Dept Gastroenterol,Shanghai Peoples Hosp 10,Sch M, Suzhou, Peoples R China

来源：

ONCOTARGETS AND THERAPY | 2015年 / 8卷

基金：

中国国家自然科学基金;

关键词：

15-deoxy-Delta(12,14)-prostaglandin J(2); hepatoma cell; ROS; apoptosis; JNK; ACTIVATED RECEPTOR-GAMMA; CANCER-CELLS; AKT; PATHWAY; TARGET; GROWTH; ROS; MECHANISMS; EXPRESSION; MIGRATION;

D O I：

10.2147/OTT.S92832

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Objective: 15-Deoxy-Delta(12,14)-prostaglandin J(2) (15d-PGJ(2)) induces reactive oxygen species (ROS)-mediated apoptosis in many malignant cells, which has not been studied in hepatoma cells. In this study, we investigated whether 15d-PGJ(2) induced apoptosis in hepatocellular carcinoma (HCC) associated with ROS. Materials and methods: The LM3, SMMC-7721, and Huh-7 HCC cell lines were treated with 15d-PGJ(2) (5-40 mu M) for 24, 48, and 72 hours. Cholecystokinin 8 was used to detect the cytotoxicity of 15d-PGJ(2). Flow cytometry, Hoechst staining, and Western blotting were used to analyze apoptosis. ROS were combined with the fluorescent probe dihydroethidium and then observed by fluorescence microscopy and flow cytometry. Activation of JNK and expression of Akt were detected by Western blotting. Results: 15d-PGJ(2) inhibited HCC cell proliferation and induced apoptosis in a dose-and time-dependent manner. Apoptosis was mainly induced via an intrinsic pathway and was ROS-dependent, and was alleviated by ROS scavengers. ROS induced JNK activation and Akt downregulation in HCC cells. Conclusion: 15d-PGJ(2) induced ROS in HCC cell lines, and inhibition of cell growth and apoptosis were partly ROS-dependent.

引用

页码：3585 / 3593

页数：9

共 37 条

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