Chronic TNFα and cAMP pre-treatment of human adipocytes alter HSL, ATGL and perilipin to regulate basal and stimulated lipolysis

被引：43
作者：
Bezaire, Veronic [1 ,2 ]
Mairal, Aline [1 ,2 ]
Anesia, Rodica [1 ,2 ]
Lefort, Corinne [1 ,2 ]
Langin, Dominique [1 ,2 ,3 ]
机构：
[1] INSERM, U858, Lab Rech Obesites, F-31432 Toulouse 4, France
[2] Univ Toulouse, UPS, Inst Med Mol Rangueil, IFR150, Toulouse, France
[3] CHU Toulouse, Biochim Lab, Inst Federatif Biol Purpan, Toulouse, France
来源：
FEBS LETTERS | 2009年 / 583卷 / 18期
基金：
加拿大自然科学与工程研究理事会;
关键词：
Hormone-sensitive lipase; Adipose triglyceride lipase; Lipolysis; TNF alpha; Forskolin; Protein kinase A; HORMONE-SENSITIVE LIPASE; ADIPOSE TRIGLYCERIDE LIPASE; TUMOR-NECROSIS-FACTOR; CHANARIN-DORFMAN-SYNDROME; 3T3-L1; ADIPOCYTES; GENE-EXPRESSION; PROTEIN TRAFFICKING; TISSUE LIPOLYSIS; LIPID DROPLETS; INSULIN;
D O I：
10.1016/j.febslet.2009.08.019
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We examined the effects of chronic TNF alpha and dibutyryl-cAMP (Db-cAMP) pre-treatment on the lipolytic machinery of human hMADS adipocytes. TNF alpha decreased adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL) protein content and triglycerides (TG)-hydrolase activity but increased basal lipolysis due to a marked reduction in perilipin (PLIN) protein content. Conversely, Db- cAMP increased ATGL and HSL protein content but prevented PLIN phosphorylation, the net result being accentuated basal lipolysis. In forskolin-stimulated conditions, TNF alpha and Db- cAMP pre-treatment decreased stimulated TG-hydrolase activity and impaired PLIN phosphorylation. Together, this resulted in a severely attenuated response to forskolin-stimulated lipolysis. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
引用
收藏
页码：3045 / 3049
页数：5
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