Real-Time PCR for Measles Virus Detection on Clinical Specimens with Negative IgM Result in Morocco

被引:13
作者
Benamar, Touria [1 ]
Tajounte, Latifa [1 ]
Alla, Amal [2 ]
Khebba, Fatima [1 ]
Ahmed, Hinda [3 ]
Mulders, Mick N. [4 ]
Filali-Maltouf, Abdelkarim [5 ]
El Aouad, Rajae [6 ]
机构
[1] Minist Hlth, Natl Inst Hyg, Rabat, Morocco
[2] Minist Hlth, Dept Epidemiol, Rabat, Morocco
[3] WHO EMRO, Dept Communicable Dis Prevent & Control, Cairo, Egypt
[4] WHO, Expanded Programme Immunizat, CH-1211 Geneva, Switzerland
[5] Univ Mohammed 5, Fac Sci, Microbiol & Mol Biol Lab, Rabat, Morocco
[6] Univ Mohamed V Souissi, Sch Med & Pharm, Rabat, Morocco
关键词
LABORATORY CONFIRMATION; MOLECULAR DIAGNOSIS; INFECTION; ELIMINATION; IDENTIFICATION; EXPERIENCE; GENOTYPES; SETTINGS; OUTBREAK; ASSAYS;
D O I
10.1371/journal.pone.0147154
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Since the confirmation of measles cases represents an important indicator regarding the performance of the measles-elimination program, the aim of this study was to evaluate the effectiveness of the routine procedures followed in Morocco for the laboratory confirmation of measles cases. Suspected cases reported between January 2010 and December 2012 were assessed for the timeliness of the sample collection, occurrence of measles clinical symptoms, and the results of the laboratory diagnoses. For 88% of the 2,708 suspected cases, a clinical specimen was collected within 7d of rash onset, of which 50% were IgM-positive and 2.6% were equivocal. The measles symptoms were reported in 91.4% of the cases; the occurrence of symptoms showed a positive association with the serological results (odds ratio [OR] = 2.9883, 95% confidence interval [CI] 2.2238-4.0157). Of the negative samples, 52% (n = 116) tested positive by real-time polymerase chain reaction (PCR). These results are in favor of using molecular detection to complement serological diagnosis in the context of measles surveillance approach in Morocco. In addition, the introduction of additional laboratory methods for differential diagnosis is required for the final classification of suspected cases with maculopapular rash and fever in the context of the measles elimination program.
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页数:10
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