Arrhythmia Susceptibility in Mice after Therapy with β-Catenin-Transduced Hematopoietic Progenitor Cells after Myocardial Ischemia/Reperfusion

被引：4

作者：

Gardiwal, Ajmal ^{[2
]}

Reissmann, Lara-Marie ^{[2
]}

Kotlarz, Daniel ^{[2
]}

Oswald, Hanno ^{[2
]}

Korte, Thomas ^{[2
]}

Landmesser, Ulf ^{[1
]}

Klein, Gunnar ^{[2
]}

Templin, Christian ^{[1
,2
]}

机构：

[1] Univ Zurich Hosp, Ctr Cardiovasc, CH-8091 Zurich, Switzerland

[2] Hannover Med Sch, Dept Cardiovasc Med, D-3000 Hannover, Germany

来源：

CARDIOLOGY | 2009年 / 114卷 / 03期

关键词：

Arrhythmia; Electrophysiology; Hematopoietic progenitor cell line; Gene transfer; SKELETAL MYOBLAST TRANSPLANTATION; ISCHEMIC-HEART FAILURE; BONE-MARROW-CELLS; VENTRICULAR-ARRHYTHMIAS; CARDIAC-FUNCTION; GENE-TRANSFER; STEM-CELLS; FOLLOW-UP; INFARCTION; RECEPTOR;

D O I：

10.1159/000228644

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Background: Hematopoietic progenitor cells (HPCs) can improve cardiac function after myocardial infarction. However, occurrence of arrhythmias is a potential limitation of cell therapy. In this study, we investigated the cardiac electrophysiological properties of ex vivo expanded HPCs, generated by beta-catenin gene transfer, after transcoronary delivery in a murine model of ischemia/reperfusion (I/R) injury. Methods and Results: To assess arrhythmia inducibility of ex vivo expanded HPCs, mice were subjected to I/R and assigned to sham operation (n = 8), I/R (n = 21) and HPC (n = 15) treatment. Six weeks later, mice were subjected to long-term electrocardiogram recording and in vivo transvenous electrophysiological study. After I/R, mice showed a significant prolongation of conduction and repolarization compared with sham-operated mice. There was a marked increase in ventricular ectopic activity in infarcted mice as compared with sham-operated mice. Cardiac electrophysiological parameters and ventricular ectopic activity were not altered in mice treated with HPCs in comparison with control I/R mice. Conclusion: Transcoronary delivery of genetically ex vivo expanded HPCs did not alter the electrophysiological properties in mice after I/R. Therefore, ex vivo beta-catenin-mediated HPC expansion may represent an attractive therapeutic option for cell transplantation treatment of myocardial infarction without electrophysiological side effects. Copyright (C) 2009 S. Karger AG, Basel

引用

页码：199 / 207

页数：9

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