Isolation of novel indole-3-acetic acid conjugates by immunoaffinity extraction

被引：80

作者：

Pencik, Ales ^{[1
,2
]}

Rolcik, Jakub ^{[1
,2
]}

Novak, Ondrej ^{[1
,2
]}

Magnus, Volker ^{[3
]}

Bartak, Petr ^{[4
]}

Buchtik, Roman ^{[1
,2
]}

Salopek-Sondi, Branka ^{[3
]}

Strnad, Miroslav ^{[1
,2
]}

机构：

[1] Palacky Univ, Fac Sci, Lab Growth Regulators, CZ-78371 Olomouc, Czech Republic

[2] Inst Expt Bot ASCR, CZ-78371 Olomouc, Czech Republic

[3] Rudjer Boskovic Inst, HR-10000 Zagreb, Croatia

[4] Palacky Univ, Fac Sci, Dept Analyt Chem, CZ-77146 Olomouc, Czech Republic

来源：

TALANTA | 2009年 / 80卷 / 02期

关键词：

Immunoaffinity extraction; High-performance liquid chromatography (HPLC); Tandem mass spectrometry (MS/MS); Indole-3-acetic acid (IAA); Auxin conjugates; Helleborus niger L; TANDEM MASS-SPECTROMETRY; SOLID-PHASE EXTRACTION; ABSCISIC-ACID; MONOCLONAL-ANTIBODIES; QUANTITATIVE-ANALYSIS; PLANT-TISSUES; CHROMATOGRAPHY; PURIFICATION; ARABIDOPSIS; CYTOKININS;

D O I：

10.1016/j.talanta.2009.07.043

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An analytical protocol for the isolation and quantification of indole-3-acetic acid (IAA) and its amino acid conjugates was developed. IAA is an important phytohormone and formation of its conjugates plays a crucial role in regulating IAA levels in plants. The developed protocol combines a highly specific immunoaffinity extraction with a sensitive and selective LC-MS/MS analysis. By using internal standards for each of the studied compounds, IAA and seven amino acid conjugates were analyzed in quantities of fresh plant material as low as 30 mg. In seeds of Helleborus niger, physiological levels of these compounds were found to range from 7.5 nmol g(-1) fresh weight (IAA) to 0.44 pmol g(-1) fresh weight (conjugate with Ala). To our knowledge, the identification of IAA conjugates with Gly, Phe and Val from higher plants is reported here for the first time. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：651 / 655

页数：5

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