Microarray analysis of microRNA expression in renal clear cell carcinoma

被引：84

作者：

Huang, Y. ^{[2
]}

Dai, Y. ^{[1
]}

Yang, J. ^{[1
]}

Chen, T. ^{[1
]}

Yin, Y. ^{[2
]}

Tang, M. ^{[2
]}

Hu, C. ^{[1
]}

Zhang, L. ^{[1
]}

机构：

[1] Jinan Univ, Shenzhen Peoples Hosp, Clin Med Coll 2, Guangzhou 518020, Guangdong, Peoples R China

[2] Chongqing Med Univ, Minist Educ, Key Lab Lab Med Diagnost, Chongqing 400016, Peoples R China

来源：

EJSO | 2009年 / 35卷 / 10期

关键词：

MicroRNA; Microarray; Renal cell carcinoma; Human; CLINICAL DEVELOPMENT; TARGETED THERAPY; GENE; RNA; INTERLEUKIN-2; INTERFERON; INDUCTION; APOPTOSIS; CANCERS; FAMILY;

D O I：

10.1016/j.ejso.2009.04.010

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Aim: To explore the microRNA (miRNA) expression in renal clear cell carcinoma (RCCC). Methods: We compared the miRNA expression profiles in 11 pairs of RCCC and adjacent nontumorous tissue (NT) from 11 RCCC patients, using a mammalian miRNA microarray containing whole human mature and precursor miRNA sequences. To verify microarray results, Northern blotting was carried out on 5 randomly selected miRNAs. Results: Totally 81 miRNAs were identified valid expression in RCCC samples, 48 of which specifically detected in RCCC samples, 17 of which detected downregulated in RCCC compared to NT sample, 2 upregulated and 14 without significant difference. MiRNAs in RCCC tissues exhibit an overall higher expression level than NT tissue. The chip results were confirmed by northern blot analysis. Conclusion: Our study may help to clarify the molecular mechanisms involved in the pathogenesis of RCCC, and miRNAs potentially serve as a novel diagnostic biomarker of RCCC. Crown Copyright (C) 2009 Published by Elsevier Ltd. All rights reserved.

引用

页码：1119 / 1123

页数：5

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