Single-Cell Sequencing Applications in the Inner Ear

被引：7
作者：
Wu, Mingxuan [1 ,2 ,3 ]
Xia, Mingyu [1 ,2 ,3 ]
Li, Wenyan [1 ,2 ,3 ]
Li, Huawei [1 ,2 ,3 ,4 ,5 ,6 ,7 ]
机构：
[1] Fudan Univ, ENT Inst, Eye & ENT Hosp, State Key Lab Med Neurobiol, Shanghai, Peoples R China
[2] Fudan Univ, Dept Otorhinolaryngol, Eye & ENT Hosp, State Key Lab Med Neurobiol, Shanghai, Peoples R China
[3] Fudan Univ, MOE Frontiers Ctr Brain Sci, Shanghai, Peoples R China
[4] Fudan Univ, Inst Biomed Sci, Shanghai, Peoples R China
[5] Fudan Univ, NHC Key Lab Hearing Med, Shanghai, Peoples R China
[6] Fudan Univ, Inst Brain Sci, Shanghai, Peoples R China
[7] Fudan Univ, Collaborat Innovat Ctr Brain Sci, Shanghai, Peoples R China
来源：
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY | 2021年 / 9卷
关键词：
single cell sequencing; hair cell; inner ear; supporting cell; spiral ganglion neuron; DNA METHYLOME LANDSCAPES; SENSORY HAIR-CELLS; MESSENGER-RNA-SEQ; DISCHARGE PATTERNS; GENE-EXPRESSION; SIGNALING PATHWAYS; MAMMALIAN COCHLEA; STEM-CELLS; REVEALS; GENOME;
D O I：
10.3389/fcell.2021.637779
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Genomics studies face specific challenges in the inner ear due to the multiple types and limited amounts of inner ear cells that are arranged in a very delicate structure. However, advances in single-cell sequencing (SCS) technology have made it possible to analyze gene expression variations across different cell types as well as within specific cell groups that were previously considered to be homogeneous. In this review, we summarize recent advances in inner ear research brought about by the use of SCS that have delineated tissue heterogeneity, identified unknown cell subtypes, discovered novel cell markers, and revealed dynamic signaling pathways during development. SCS opens up new avenues for inner ear research, and the potential of the technology is only beginning to be explored.
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页数：12
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