Design of thermally responsive, recombinant polypeptide carriers for targeted drug delivery

被引：215

作者：

Chilkoti, A ^{[1
]}

Dreher, MR ^{[1
]}

Meyer, DE ^{[1
]}

机构：

[1] Duke Univ, Dept Biomed Engn, Durham, NC 27708 USA

来源：

ADVANCED DRUG DELIVERY REVIEWS | 2002年 / 54卷 / 08期

关键词：

artificial polypeptide; genetically encoded synthesis; elastin-like polypeptide; thermally responsive; drug delivery; thermal targeting; cancer therapy; block copolymers; nanoparticles; micelles;

D O I：

10.1016/S0169-409X(02)00060-1

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

In this article, we review recombinant DNA methods for the design and synthesis of amino acid-based biopolymers, and briefly summarize an approach, recursive directional ligation (RDL), that we have employed to synthesize oligomeric genes for such biopolymers. We then describe our ongoing research in the use of RDL to synthesize recombinant polypeptide carriers for the targeted delivery of radionuclides, chemotherapeutics and biomolecular therapeutics to tumors. The targeted delivery system uses a thermally responsive, elastin-like polypeptide (ELP) as the drug carrier to enhance the localization of ELP-drug conjugates within a solid tumor that is heated by regional hyperthermia. In the context of this drug delivery application, we discuss the design of ELPs and their recombinant synthesis, which enables the molecular weight and the thermal properties of the polypeptide to be precisely controlled. Finally, our results pertaining to the in vivo targeting of tumors with ELPs are briefly summarized. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：1093 / 1111

页数：19

共 61 条

[11] Effect of molecular architecture of hydrophobically modified poly(N-isopropylacrylamide) on the formation of thermoresponsive core-shell micellar drug carriers [J].