Acoustofluidic rotational tweezing enables high-speed contactless morphological phenotyping of zebrafish larvae

被引：72

作者：

Chen, Chuyi ^{[1
]}

Gu, Yuyang ^{[1
]}

Philippe, Julien ^{[2
]}

Zhang, Peiran ^{[1
]}

Bachman, Hunter ^{[1
]}

Zhang, Jinxin ^{[1
]}

Mai, John ^{[3
]}

Rufo, Joseph ^{[1
]}

Rawls, John F. ^{[4
]}

Davis, Erica E. ^{[2
,5
,6
]}

Katsanis, Nicholas ^{[2
,5
,6
]}

Huang, Tony Jun ^{[1
]}

机构：

[1] Duke Univ, Dept Mech Engn & Mat Sci, Durham, NC 27706 USA

[2] Duke Univ Med Ctr, Ctr Human Dis Modeling, Durham, NC USA

[3] Univ Southern Calif, Alfred E Mann Inst Biomed Engn, Los Angeles, CA 90007 USA

[4] Duke Univ, Dept Mol Genet & Microbiol, Durham, NC USA

[5] Ann & Robert H Lurie Childrens Hosp Chicago, Stanley Manne Childrens Res Inst, Adv Ctr Translat & Genet Med ACT GeM, Chicago, IL USA

[6] Northwestern Univ, Dept Pediat, Feinberg Sch Med, Chicago, IL 60611 USA

来源：

NATURE COMMUNICATIONS | 2021年 / 12卷 / 01期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

ALCOHOL EXPOSURE; GENE-EXPRESSION; HUMAN-DISEASE; SINGLE CELLS; MANIPULATION; MODEL; FISH; TOMOGRAPHY; TOOLS; RECONSTRUCTION;

D O I：

10.1038/s41467-021-21373-3

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Modern biomedical research and preclinical pharmaceutical development rely heavily on the phenotyping of small vertebrate models for various diseases prior to human testing. In this article, we demonstrate an acoustofluidic rotational tweezing platform that enables contactless, high-speed, 3D multispectral imaging and digital reconstruction of zebrafish larvae for quantitative phenotypic analysis. The acoustic-induced polarized vortex streaming achieves contactless and rapid (similar to 1s/rotation) rotation of zebrafish larvae. This enables multispectral imaging of the zebrafish body and internal organs from different viewing perspectives. Moreover, we develop a 3D reconstruction pipeline that yields accurate 3D models based on the multi-view images for quantitative evaluation of basic morphological characteristics and advanced combinations of metrics. With its contactless nature and advantages in speed and automation, our acoustofluidic rotational tweezing system has the potential to be a valuable asset in numerous fields, especially for developmental biology, small molecule screening in biochemistry, and pre-clinical drug development in pharmacology.

引用

页数：13

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