A new parvovirus genotype persistent in human skin

被引：116

作者：

Hokynar, K ^{[1
]}

Söderlund-Venermo, M

Pesonen, M

Ranki, A

Kiviluoto, O

Partio, EK

Hedman, K

机构：

[1] Haartman Inst, Dept Virol, FIN-00290 Helsinki, Finland

[2] Univ Helsinki, Cent Hosp, FIN-00290 Helsinki, Finland

[3] HUCH, Dept Dermatol, FIN-00014 Helsinki, Finland

[4] Cent Mil Hosp, FIN-00300 Helsinki, Finland

[5] Dextra Med Ctr, FIN-00350 Helsinki, Finland

来源：

VIROLOGY | 2002年 / 302卷 / 02期

基金：

芬兰科学院;

关键词：

DNA; erythrovirus; genotype; parvovirus B19; persistence; skin;

D O I：

10.1006/viro.2002.1673

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Parvovirus B19 is the exclusive human pathogen of the Erythrovirus genus. In classical view, the B19 DNA sequence shows little variability, with no disease-specific or tissue type specific associations. We examined skin biopsies from patients with B19-unrelated skin disease or from constitutionally healthy adults by polymerase chain reaction assays for four different genomic regions of the B19 virus. Sequencing showed that the skin-derived viral DNA differed within the protein-coding region from the B19 reference sequences by 10.8% and from the V9 variant by 8.6% and within the noncoding region (covering nucleotides 189-435 of the promoter region) by 26.5 and 17.2%, respectively. Despite this sequence difference, the promoter region was shown by a luciferase gene expression assay to be biologically active. We have detected a new B19 virus genotype, K71, which differs extensively from the known B19-virus genotypes and is persistently carried in human skin. (C) 2002 Elsevier Science (USA).

引用

页码：224 / 228

页数：5

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