A new parvovirus genotype persistent in human skin

被引：116
作者：
Hokynar, K [1 ]
Söderlund-Venermo, M
Pesonen, M
Ranki, A
Kiviluoto, O
Partio, EK
Hedman, K
机构：
[1] Haartman Inst, Dept Virol, FIN-00290 Helsinki, Finland
[2] Univ Helsinki, Cent Hosp, FIN-00290 Helsinki, Finland
[3] HUCH, Dept Dermatol, FIN-00014 Helsinki, Finland
[4] Cent Mil Hosp, FIN-00300 Helsinki, Finland
[5] Dextra Med Ctr, FIN-00350 Helsinki, Finland
来源：
VIROLOGY | 2002年 / 302卷 / 02期
基金：
芬兰科学院;
关键词：
DNA; erythrovirus; genotype; parvovirus B19; persistence; skin;
D O I：
10.1006/viro.2002.1673
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Parvovirus B19 is the exclusive human pathogen of the Erythrovirus genus. In classical view, the B19 DNA sequence shows little variability, with no disease-specific or tissue type specific associations. We examined skin biopsies from patients with B19-unrelated skin disease or from constitutionally healthy adults by polymerase chain reaction assays for four different genomic regions of the B19 virus. Sequencing showed that the skin-derived viral DNA differed within the protein-coding region from the B19 reference sequences by 10.8% and from the V9 variant by 8.6% and within the noncoding region (covering nucleotides 189-435 of the promoter region) by 26.5 and 17.2%, respectively. Despite this sequence difference, the promoter region was shown by a luciferase gene expression assay to be biologically active. We have detected a new B19 virus genotype, K71, which differs extensively from the known B19-virus genotypes and is persistently carried in human skin. (C) 2002 Elsevier Science (USA).
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收藏
页码：224 / 228
页数：5
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