Rapid quantification of free and glucuronidated THCCOOH in urine using coated well plates and LC-MS/MS analysis

被引：11

作者：

Hadener, Marianne ^{[1
]}

Weinmann, Wolfgang ^{[1
]}

van Staveren, Dave R. ^{[2
]}

Konig, Stefan ^{[1
]}

机构：

[1] Univ Bern, Inst Forens Med, Buhlstr 20, CH-3012 Bern, Switzerland

[2] Tecan Schweiz AG, Seestr 103, CH-8708 Mannedorf, Switzerland

来源：

BIOANALYSIS | 2017年 / 9卷 / 05期

关键词：

column-switching LC-MS/MS; THCCOOH; THCCOOH-glucuronide; TANDEM MASS-SPECTROMETRY; SOLID-PHASE EXTRACTION; WHOLE-BLOOD; CHROMATOGRAPHY; CANNABINOIDS; ACID; PLASMA; DRUG; CONFIRMATION; METABOLITES;

D O I：

10.4155/bio-2016-0281

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Aim: Generally, urine drug testing for cannabis abuse involves measuring total concentrations of 11-nor-9-carboxy-Delta(9)- tetrahydrocannabinol (THCCOOH) obtained by enzymatic and/or alkaline hydrolysis of THCCOOH-glucuronide. As hydrolysis can be inconsistent and incomplete, direct measurement of the two metabolites is preferable. Methodology & results: We developed a high-throughput LC-MS/MS method for simultaneous quantification of free and glucuronidated THCCOOH in urine using coated 96-well plates for analyte extraction and column-switching chromatography. Excellent separation of the two analytes was achieved within 2.5 min, with linear ranges from 5 to 2000 mu g/l for THCCOOH and from 10 to 4000 mu g/l for THCCOOH-glucuronide. Conclusion: The method was successfully validated and applied to authentic urine samples from cannabis consumers, demonstrating its applicability for routine cannabinoid testing.

引用

页码：485 / 496

页数：12