Osteogenic Differentiation of Human Bone Marrow Mesenchymal Stem Cells Seeded on Melt Based Chitosan Scaffolds for Bone Tissue Engineering Applications

被引：100

作者：

Costa-Pinto, Ana R. ^{[1
,2
]}

Correlo, Vitor M. ^{[1
,2
]}

Sol, Paula C. ^{[1
,2
]}

Bhattacharya, Mrinal ^{[3
]}

Charbord, Pierre ^{[4
]}

Delorme, Bruno ^{[4
]}

Reis, Rui L. ^{[1
,2
]}

Neves, Nuno M. ^{[1
,2
]}

机构：

[1] Univ Minho, Headquarters European Inst Excellence Tissue Engn, Dept Polymer Engn, 3Bs Res Grp Biomat Biodegradables & Biomimet, P-4806909 Caidas Das Taipas, Guimaraes, Portugal

[2] PT Govt Associated Lab, Inst Biotechnol & Bioengn, IBB, Guimaraes, Portugal

[3] Univ Minnesota, Dept Biosyst Engn, St Paul, MN 55108 USA

[4] Fac Med Tours, INSERM, Equipe ESPRI, EA3855, F-37032 Tours 1, France

来源：

BIOMACROMOLECULES | 2009年 / 10卷 / 08期

关键词：

FIBER-MESH SCAFFOLDS; OF-THE-ART; IN-VITRO; BIODEGRADABLE POLYMERS; POTENTIAL APPLICATION; HYDROXYAPATITE; ADHESION; BIOCOMPATIBILITY; PROLIFERATION; PERFORMANCE;

D O I：

10.1021/bm9000102

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The purpose of this study was to evaluate the growth patterns and osteogenic differentiation of human bone marrow mesenchymal stein cells (hBMSCs) when seeded onto new biodegradable chitosan/polyester scaffolds. Scaffolds were obtained by melt blending chitosan with poly(butylene succinate) in a proportion of 50% (wt) each and further used to produce a fiber mesh scaffold. hBMSCs were seeded on those structures and cultured for 3 weeks under osteogenic conditions. Cells were able to reduce MTS and demonstrated increasing metabolic rates over time. SEM observations showed cell colonization at the surface as well as within the scaffolds. The presence of mineralized extracellular matrix (ECM) was successfully demonstrated by peaks corresponding to calcium and phosphorus elements detected in the EDS analysis. A further confirmation was obtained when carbonate and phosphate group peaks were identified in Fourier Transformed Infrared (FTIR) spectra. Moreover, by reverse transcriptase (RT)-PCR analysis, it was observed the expression of osteogenic gene markers, namely, Runt related transcription factor 2 (Runx2), type I collagen, bone sialoprotein (BSP), and osteocalcin. Chitosan-PBS (Ch-PBS) biodegradable scaffolds support the proliferation and osteogenic differentiation of hBMSCs cultured at their surface in vitro, enabling future in vivo testing for the development of bone tissue engineering therapies.

引用

页码：2067 / 2073

页数：7

共 44 条

[31] Assessment of the Suitability of Chitosan/PolyButylene Succinate Scaffolds Seeded with Mouse Mesenchymal Progenitor Cells for a Cartilage Tissue Engineering Approach[J]. Oliveira, Joao T.;Correlo, Vitor M.;Sol, Paula C.;Costa-Pinto, Ana R.;Malafaya, Patricia B.;Salgado, Antonio J.;Bhattacharya, Mrinal;Charbord, Pierre;Neves, Nuno M.;Reis, Rui L. TISSUE ENGINEERING PART A, 2008(10)
[32] Novel hydroxyapatite/chitosan bilayered scaffold for osteochondral tissue-engineering applications: Scaffold design and its performance when seeded with goat bone marrow stromal cells[J]. Oliveira, Joaquim M.;Rodrigues, Marcia T.;Silva, Simone S.;Malafaya, Patricia B.;Gomes, Manuela E.;Viegas, Carlos A.;Dias, Isabel R.;Azevedo, Jorge T.;Mano, Joao F.;Reis, Rui L. BIOMATERIALS, 2006(36)
[33] OWEN M, 1988, J CELL SCI, P63
[34] PETILLO O, 1994, BIOMATERIALS, V15, P1215
[35] Characterization of hydroxyapatite and carbonated apatite by photo acoustic FTIR spectroscopy[J]. Rehman, I;Bonfield, W. JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, 1997(01)
[36] Characterization of two biodegradable polymers of potential application within the biomaterials field[J]. Reis, RL;Cunha, AM. JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, 1995(12)
[37] Bone tissue engineering: State of the art and future trends[J]. Salgado, AJ;Coutinho, OP;Reis, RL. MACROMOLECULAR BIOSCIENCE, 2004(08)
[38] Novel starch-based scaffolds for bone tissue engineering: Cytotoxicity, cell culture, and protein expression[J]. Salgado, AJ;Coutinho, OP;Reis, RL. TISSUE ENGINEERING, 2004(3-4)
[39] Preliminary study on the adhesion and proliferation of human osteoblasts on starch-based scaffolds[J]. Salgado, AJ;Gomes, ME;Chou, A;Coutinho, OP;Reis, RL;Hutmacher, DW. MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS, 2002(1-2)
[40] Chitosan sponges as tissue engineering scaffolds for bone formation[J]. Seol, YJ;Lee, JY;Park, YJ;Lee, YM;Young-Ku;Rhyu, IC;Lee, SJ;Han, SB;Chung, CP. BIOTECHNOLOGY LETTERS, 2004(13)

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