Reduced skeletal muscle mitochondrial respiration and improved glucose metabolism in nondiabetic obese women during a very low calorie dietary intervention leading to rapid weight loss

被引：61

作者：

Rabol, Rasmus ^{[1
,2
]}

Svendsen, Pernille F. ^{[3
]}

Skovbro, Mette ^{[1
]}

Boushel, Robert ^{[1
]}

Haugaard, Steen B. ^{[4
]}

Schjerling, Peter ^{[1
,5
]}

Schrauwen, Patrick ^{[6
,7
]}

Hesselink, Matthijs K. C. ^{[6
,7
]}

Nilas, Lisbeth ^{[3
]}

Madsbad, Sten ^{[2
]}

Dela, Flemming ^{[1
]}

机构：

[1] Univ Copenhagen, Fac Hlth Sci, Dept Biomed Sci, Ctr Healthy Aging, DK-2200 Copenhagen, Denmark

[2] Univ Copenhagen, Hvidovre Univ Hosp, Dept Endocrinol, DK-2650 Hvidovre, Denmark

[3] Univ Copenhagen, Hvidovre Univ Hosp, Dept Gynecol & Obstet, DK-2650 Hvidovre, Denmark

[4] Univ Copenhagen, Hvidovre Univ Hosp, Clin Res Ctr, DK-2650 Hvidovre, Denmark

[5] Bispebjerg Hosp, Inst Sports Med, DK-2200 Copenhagen, Denmark

[6] Maastricht Univ, Dept Human Biol, NL-6200 MD Maastricht, Netherlands

[7] Maastricht Univ, Dept Movement Sci, NL-6200 MD Maastricht, Netherlands

来源：

METABOLISM-CLINICAL AND EXPERIMENTAL | 2009年 / 58卷 / 08期

关键词：

INSULIN-RESISTANCE; THYROID-HORMONES; FATTY-ACIDS; CAPACITY; PHOSPHORYLATION; SENSITIVITY; DYSFUNCTION; MECHANISMS; EXPRESSION; TOLERANCE;

D O I：

10.1016/j.metabol.2009.03.014

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Reduced oxidative capacity of skeletal muscle has been proposed to lead to accumulation of intramyocellular triglyceride (IMTG) and insulin resistance. We have measured mitochondrial respiration before and after a 10% low-caloric-induced weight loss ill young obese women to examine the relationship between mitochondrial function, IMTG, and insulin resistance. Nine obese women (age, 32.3 years [SD, 3.0]; body mass index, 33.4 kg/m(2) [SD, 2.6]) completed a 53-day (SE, 3.8) very low calorie diet (VLCD) of 500 to 600 kcal/d without altering physical activity. The target of the intervention was a 10% weight loss; and measurements of mitochondrial respiration, IMTG, respiratory exchange ratio, citrate synthase activity, mitochondrial DNA copy number, plasma insulin, 2-hour oral glucose tolerance test, and free fatty acids were performed before and after weight loss. Mitochondrial respiration was measured in permeabilized muscle fibers using high-resolution respirometry. Average weight loss was 11.5% (P<.05), but the levels of IMTG remained unchanged. Fasting plasma glucose, plasma insulin homeostasis model assessment of insulin resistance, and insulin sensitivity index (composite) obtained during 2-hour oral glucose tolerance test improved significantly. Mitochondrial respiration per milligram tissue decreased by approximately 25% (P<.05), but citrate synthase activity and rnitochondrial DNA copy number remained unchanged. Respiratory exchange ratio decreased from 0.87 (SE, 0.01) to 0.79 (SE, 0.02) (P<.05) as a sign of increased whole-body fat oxidation. Markers Of insulin sensitivity improved after the very low calorie diet; but mitochondrial function decreased, and IMTG remained unchanged. Our results do not support a direct relationship between mitochondrial function and insulin resistance in young obese women and do not support a direct relationship between IMTG and insulin sensitivity in young obese women during weight loss. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：1145 / 1152

页数：8

共 39 条

[31] The effect of weight reduction on skeletal muscle UCP2 and UCP3 mRNA expression and UCP3 protein content in Type II diabetic subjects [J].