Quantum-Optically Enhanced STORM (QUEST) for Multi-Emitter Localization

被引：9

作者：

Assmann, Marc ^{[1
]}

机构：

[1] Tech Univ Dortmund, Expt Phys 2, D-44227 Dortmund, Germany

来源：

SCIENTIFIC REPORTS | 2018年 / 8卷

关键词：

SINGLE-MOLECULE; SUPERRESOLUTION; FLUORESCENCE; MICROSCOPY; RESOLUTION; BLINKING; LIMIT;

D O I：

10.1038/s41598-018-26271-1

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Super-resolution imaging has introduced new capabilities to investigate processes at the nanometer scale by optical means. However, most super-resolution techniques require either sparse excitation of few emitters or analysis of high-order cumulants in order to identify several emitters in close vicinity. Here, we present an approach that draws upon methods from quantum optics to perform localization super-resolution imaging of densely packed emitters and determine their number automatically: Quantum-optically enhanced STORM (QUEST). By exploiting normalized photon correlations, we predict a localization precision below 30 nm or better even for closely spaced emitter up to a density of 125 emitters per mu m at photon emission rates of 10(5) photons per second and emitter. Our technique does not require complex experimental arrangements and relies solely on spatially resolved time streams of photons and subsequent data analysis.

引用

页数：12

共 37 条

[1] Super-resolution of dense nanoscale emitters beyond the diffraction limit using spatial and temporal information [J].

Barsic, Anthony ;

Piestun, Rafael .

APPLIED PHYSICS LETTERS, 2013, 102 (23)

[2] PHOTON ANTIBUNCHING IN THE FLUORESCENCE OF A SINGLE DYE MOLECULE TRAPPED IN A SOLID [J].

BASCHE, T ;

MOERNER, WE ;

ORRIT, M ;

TALON, H .

PHYSICAL REVIEW LETTERS, 1992, 69 (10) :1516-1519

[3] The ATLAS Tile Calorimeter digitizer [J].

Berglund, S. ;

Bohm, C. ;

Engstrom, M. ;

Eriksson, D. ;

Holmgren, S-O. ;

Jon-And, K. ;

Lesser, J. ;

Sellden, B. ;

Ramstedt, M. ;

Silverstein, S. ;

Sjolin, J. .

JOURNAL OF INSTRUMENTATION, 2008, 3 (01)

[4] Imaging intracellular fluorescent proteins at nanometer resolution [J].

Betzig, Eric ;

Patterson, George H. ;

Sougrat, Rachid ;

Lindwasser, O. Wolf ;

Olenych, Scott ;

Bonifacino, Juan S. ;

Davidson, Michael W. ;

Lippincott-Schwartz, Jennifer ;

Hess, Harald F. .

SCIENCE, 2006, 313 (5793) :1642-1645

[5] A quantum imager for intensity correlated photons [J].

Boiko, D. L. ;

Gunther, N. J. ;

Brauer, N. ;

Sergio, M. ;

Niclass, C. ;

Beretta, G. B. ;

Charbon, E. .

NEW JOURNAL OF PHYSICS, 2009, 11

[6] Architecture and applications of a high resolution gated SPAD image sensor [J].

Burri, Samuel ;

Maruyama, Yuki ;

Michalet, Xavier ;

Regazzoni, Francesco ;

Bruschini, Claudio ;

Charbon, Edoardo .

OPTICS EXPRESS, 2014, 22 (14) :17573-17589

[7]

Cox S, 2012, NAT METHODS, V9, P195, DOI [10.1038/NMETH.1812, 10.1038/nmeth.1812]

[8] Quantum Statistical Imaging of Particles without Restriction of the Diffraction Limit [J].

Cui, Jin-Ming ;

Sun, Fang-Wen ;

Chen, Xiang-Dong ;

Gong, Zhao-Jun ;

Guo, Guang-Can .

PHYSICAL REVIEW LETTERS, 2013, 110 (15)

[9] Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI) [J].

Dertinger, T. ;

Colyer, R. ;

Iyer, G. ;

Weiss, S. ;

Enderlein, J. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (52) :22287-22292

[10] Learning a Deep Convolutional Network for Image Super-Resolution [J].

Dong, Chao ;

Loy, Chen Change ;

He, Kaiming ;

Tang, Xiaoou .

COMPUTER VISION - ECCV 2014, PT IV, 2014, 8692 :184-199

← 1 2 3 4 →