Eimeria tenella:: Cloning of a novel Eimeria tenella cDNA encoding a protein related to rhomboid family from F2 hybrid strain

被引：14

作者：

Li, Jianhua ^{[1
]}

Zhang, Xichen ^{[1
]}

Liu, Quan ^{[1
]}

Yin, Jigang ^{[1
]}

Yang, Ju ^{[1
]}

机构：

[1] Jilin Univ, Dept Prevent Vet Med, Changchun 130062, Peoples R China

来源：

EXPERIMENTAL PARASITOLOGY | 2006年 / 113卷 / 04期

基金：

中国国家自然科学基金;

关键词：

coccidiosis; cDNA; Eimeria tenella; hybrid strain; rhomboid; CD; DAB; ELISA; ESTs; E; tenella; ETRH01; HRP; IPTG; McAb; mRNA; ORF; P56-24; PCR; p-ETRH01; P; spp; RT; T; gondii; TMB;

D O I：

10.1016/j.exppara.2006.01.011

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

A novel cDNA sequence with an open reading frame of 774 bp from Eimeria tenella F2 hybrid strain (ETRH01) was isolated from a lamda cDNA library with a monoclonal antibody against sporozoite. Analysis of the genomic sequence suggests that this is an intronless gene. The deduced protein sequence has 257 amino acids with a calculated molecular weight of 28.349 kDa and an isoelectric point of 8.56. Sequence analysis revealed seven transmembrane domains and a rhomboid domain within the protein. RT-PCR result indicates that this gene was expressed in all of the five E. tenella isolates analyzed. To further study the role of this novel gene in the life cycle of E. tenella, ETRH01 was successfully expressed using pET28b(+) expression system. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：215 / 220

页数：6

共 17 条

[1] Recent advances in biology and immunobiology of Eimeria species and in diagnosis and control of infection with these coccidian parasites of poultry [J].

Allen, PC ;

Fetterer, RH .

CLINICAL MICROBIOLOGY REVIEWS, 2002, 15 (01) :58-+

[2]

Bashar A. E., 2003, International Journal of Poultry Science, V2, P133

[3] Vaccination against Eimeria tenella infection using a fraction of E-tenella sporozoites selected by the capacity to activate T cells [J].

Breed, DGJ ;

Schetters, TPM ;

Verhoeven, NAP ;

Boot-Groenink, A ;

Dorrestein, J ;

Vermeulen, AN .

INTERNATIONAL JOURNAL FOR PARASITOLOGY, 1999, 29 (08) :1231-1240

[4] A spatially localized rhomboid protease cleaves cell surface adhesins essential for invasion by Toxoplasma [J].

Brossier, F ;

Jewett, TJ ;

Sibley, LD ;

Urban, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (11) :4146-4151

[5] Fine architecture of bacterial inclusion bodies [J].

Carrió, MM ;

Cubarsi, R ;

Villaverde, A .

FEBS LETTERS, 2000, 471 (01) :7-11

[6] A new release on life: emerging concepts in proteolysis and parasite invasion [J].

Carruthers, VB ;

Blackman, MJ .

MOLECULAR MICROBIOLOGY, 2005, 55 (06) :1617-1630

[7] PRODUCTION OF MONOCLONAL-ANTIBODIES BY HYBRIDOMAS SENSITIZED TO SPOROZOITES OF PLASMODIUM-BERGHEI [J].

DANFORTH, HD ;

CAMPBELL, GH ;

LEEF, MF ;

BEAUDOIN, RL .

JOURNAL OF PARASITOLOGY, 1982, 68 (06) :1029-1033

[8] SPECIFICITY AND CROSSREACTIVITY OF IMMUNE SERUM AND HYBRIDOMA ANTIBODIES TO VARIOUS SPECIES OF AVIAN COCCIDIA [J].

DANFORTH, HD ;

AUGUSTINE, PC .

POULTRY SCIENCE, 1983, 62 (11) :2145-2151

[9] Role of proteases in host cell invasion by Toxoplasma gondii and other apicomplexa [J].

Kim, K .

ACTA TROPICA, 2004, 91 (01) :69-81

[10]

Koonin EV., 2003, GENOME BIOL, V4, pR19, DOI DOI 10.1186/GB-2003-4-3-R19

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