Molecular analysis of the regulation of nisin immunity

被引:42
作者
Dodd, HM
Horn, N
Chan, WC
Giffard, CJ
Bycroft, BW
Roberts, GCK
Gasson, MJ
机构
[1] UNIV NOTTINGHAM, DEPT PHARMACEUT SCI, NOTTINGHAM NG7 2RD, ENGLAND
[2] UNIV YORK, DEPT BIOL, YORK YO1 5DD, N YORKSHIRE, ENGLAND
[3] UNIV LEICESTER, DEPT BIOCHEM, LEICESTER LE1 7RH, LEICS, ENGLAND
[4] UNIV LEICESTER, BIOL NMR CTR, LEICESTER LE1 7RH, LEICS, ENGLAND
来源
MICROBIOLOGY-SGM | 1996年 / 142卷
关键词
nisin; immunity; regulation; induction; Lactococcus lactis;
D O I
10.1099/00221287-142-9-2385
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The genetic determinants controlling immunity to nisin are coordinately regulated, along with biosynthesis genes, in response to an environmental signal, nisin or a nisin analogue. The nisR gene product, the putative response regulator of nisin biosynthesis, was found to be a vital component of this induction mechanism. This protein forms part of a two-component regulatory system which controls the expression of genes involved in nisin immunity and biosynthesis. Analysis of the structural requirements of the external signal, throughout the molecule also affected its induction capacity. The production using nisin fragments and engineered nisin variants, indicated that the 12 amino-terminal residues of the molecule are a minimum requirement for induction, with an intact ring A being an essential component. Changes of certain variant nisins by engineered lactococcal strains is reduced in parallel with the strains' immunity to nisin. This can be attributed to inefficient induction by the variant molecule. Treating growing cultures with nisin restored full immunity and maximized the yields of nisin variants by the producer strains.
引用
收藏
页码:2385 / 2392
页数:8
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