Application of L-cysteine capped core-shell CdTe/ZnS nanoparticles as a fluorescence probe for cephalexin

被引：33

作者：

Li, Li ^{[1
]}

Zhang, Qiaolin ^{[1
]}

Ding, Yaping ^{[1
]}

Cai, Xiaoyong ^{[1
]}

Gu, Shuqing ^{[1
]}

Cao, Zhiyuan ^{[1
]}

机构：

[1] Shanghai Univ, Dept Chem, Shanghai 200444, Peoples R China

来源：

ANALYTICAL METHODS | 2014年 / 6卷 / 08期

基金：

上海市自然科学基金; 中国国家自然科学基金;

关键词：

LIQUID-CHROMATOGRAPHIC METHOD; CDS QUANTUM DOTS; IMMUNOANALYSIS SYSTEM; ZNS NANOPARTICLES; HUMAN PLASMA; CEFALEXIN; PHOTOLUMINESCENCE; SERUM; IONS; MILK;

D O I：

10.1039/c4ay00094c

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this paper, a novel method was developed for rapid and quantitative determination of cephalexin on the basis of the fluorescence quenching of L-cysteine capped core-shell CdTe/ZnS nanoparticles (NPs). The functionalized CdTe/ZnS NPs were successfully synthesized in aqueous solution. L-Cysteine capped CdTe/ZnS NPs were characterized by the means of X-ray diffraction (XRD), transmission electron microscopy (TEM) and Fourier transform infrared (FTIR) spectroscopy. Experimental results displayed that the fluorescence intensity of CdTe/ZnS NPs reduced in the presence of cephalexin due to interaction between L-cysteine capped CdTe/ZnS NPs and cephalexin, with the emission wavelength at about 665 nm. Under optimal conditions, the relative fluorescence intensity was linearly proportional to the concentration of cephalexin ranging from 3.4 x 10(-6) to 1.0 x 10(-4) M with a detection limit of 0.83 x 10(-6) M. The L-cysteine capped CdTe/ZnS NPs fluorescence probe showed an obvious and good response to cephalexin, and the result was also satisfactory when it was applied to analyzing cephalexin in a real sample.

引用

页码：2715 / 2721

页数：7