Generation of monoclonal antibodies against human regulatory T cells

被引：5

作者：

Becker, Christian ^{[1
]}

Hoschuetzky, Heinz ^{[2
]}

Rist, Wolfgang ^{[3
]}

Lenter, Martin ^{[3
]}

Schneider, Franz-Joseph ^{[3
]}

Jonuleit, Helmut ^{[1
]}

机构：

[1] Johannes Gutenberg Univ Mainz, Univ Med Ctr, Dept Dermatol, D-55101 Mainz, Germany

[2] NanoTools Antikorpertech GmbH, D-79331 Teningen, Germany

[3] Boehringer Ingelheim Pharma GmbH & Co KG, D-88400 Biberach, Germany

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2010年 / 353卷 / 1-2期

关键词：

Regulatory T cells; Surface receptors; Monoclonal antibodies; IMMUNOLOGICAL SELF-TOLERANCE; MASS-SPECTROMETRY; PERIPHERAL-BLOOD; HELPER-CELLS; EXPRESSION; FOXP3; IDENTIFICATION; SUPPRESSION; CD127; CD25;

D O I：

10.1016/j.jim.2010.01.002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Natural CD4(+)CD25(+)Foxp3(+) regulatory T cells (Tregs) control the activation of the immune system and therefore have become a major area of research in immunology. The generation of monoclonal antibodies against human Tregs offers the possibility to discover novel Treg-specific or Treg-associated surface markers and to identify targets for a therapeutic modulation of Tregs. Here we present a methodology optimized to efficiently induce and select mAb against human Tregs by repeated immunization of mice with Tregs from a single donor and a differential two-step flow cytometry-based hybridoma screening procedure. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：62 / 70

页数：9

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