Effect of Cell Concentration on the Acylation of Penicillin G Enzymatic Reaction in Immobilized Cells

被引：0

作者：

Barin, M.

Otadi, M. ^{[2
]}

Khorasheh, F. ^{[1
]}

Kheirolomoom, A. ^{[3
]}

机构：

[1] Sharif Univ Technol, Dept Chem & Petr Engn, Tehran, Iran

[2] Islamic Azad Univ, Biotechnol Grp, Tehran Cent Branch, Tehran, Iran

[3] Univ Calif Davis, Dept Chem Engn, Davis, CA 95616 USA

来源：

SCIENTIA IRANICA TRANSACTION C-CHEMISTRY AND CHEMICAL ENGINEERING | 2009年 / 16卷 / 01期

关键词：

E-coli; Immobilized cells; Penicillin G acylation; Cell distribution; G ACYLASE; WHOLE-CELL;

D O I：

暂无

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

E. coli was immobilized by entrapment within calcium alginate beads using cell suspensions of different concentrations. The immobilization procedure resulted in beads with a homogeneous cell distribution, referred to as a Non-overlapping Cell (NC) configuration. If cells within the beads were allowed to grow, the NC configuration would transform into a Clustered Cell (CC) configuration as a result of cell growth. Enzyme activity and substrate conversion were obtained for NC and CC configurations of different cell density, using penicillin G acylation by penicillin G acylase, to produce 6-amino penicillanic acid. Enzyme activity and conversion were found to depend on both cell concentration and configuration. Beads with NC configuration resulted in higher enzyme activity and conversion compared with CC configurations of similar cell density.

引用

页码：69 / 73

页数：5

共 15 条

[1] STUDIES ON IMPROVED TECHNIQUES FOR IMMOBILIZING AND STABILIZING PENICILLIN AMIDASE ASSOCIATED WITH ESCHERICHIA-COLI-CELLS [J].