Expression Patterns of Circular RNAs in High Quality and Poor Quality Human Spermatozoa

被引：42

作者：

Chioccarelli, Teresa ^{[1
]}

Manfrevola, Francesco ^{[1
]}

Ferraro, Bruno ^{[2
]}

Sellitto, Carolina ^{[2
]}

Cobellis, Gilda ^{[1
]}

Migliaccio, Marina ^{[1
]}

Fasano, Silvia ^{[1
]}

Pierantoni, Riccardo ^{[1
]}

Chianese, Rosanna ^{[1
]}

机构：

[1] Univ Campania L Vanvitelli, Dipartimento Med Sperimentale, Naples, Italy

[2] Presidio Osped Marcianise, UOSD Fisiopatol Riproduz, Caserta, Italy

来源：

FRONTIERS IN ENDOCRINOLOGY | 2019年 / 10卷

关键词：

circRNAs; spermatozoa; miRNAs; sperm quality; fertilization; embryo development; IDENTIFICATION; MYOINOSITOL; GENES;

D O I：

10.3389/fendo.2019.00435

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Circular RNAs (circRNAs) are expressed in human testis and seminal plasma. Until today, there is missing information about a possible payload of circRNAs in human spermatozoa (SPZ). With this in mind, we carried out a circRNA microarray identifying a total of 10.726 transcripts, 28% novel based and 84.6% with exonic structure; their potential contribution in molecular pathways was evaluated by KEGG analysis. Whether circRNAs may be related to SPZ quality was speculated evaluating two different populations of SPZ (A SPZ = good quality, B SPZ = low quality), separated on the basis of morphology and motility parameters, by Percoll gradient. Thus, 148 differentially expressed (DE)-circRNAs were identified and the expression of selected specific SPZ-derived circRNAs was evaluated in SPZ head/tail-enriched preparations, to check the preservation of these molecules during SPZ maturation and their transfer into oocyte during fertilization. Lastly, circRNA/miRNA/mRNA network was built by bioinformatics approach.

引用

页数：14

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