IMMOBILIZATION OF LACCASE IN MICROPARTICLES OF CHITOSAN OBTAINED BY SPRAY DRYING AND USED IN THE BIOSENSORS CONSTRUCTION.

被引：8
作者：
de Oliveira, Ines Rosane Welter Zwirtes [1 ]
Fatibello-Filho, Orlando [1 ]
Fernandes, Suellen Cadorin [2 ]
Vieira, Iolanda Cruz [2 ]
机构：
[1] Univ Fed Sao Carlos, Dept Quim, BR-13560970 Sao Carlos, SP, Brazil
[2] Univ Fed Santa Catarina, Dept Quim, BR-88040900 Florianopolis, SC, Brazil
来源：
QUIMICA NOVA | 2009年 / 32卷 / 05期
关键词：
spray dryer; microparticles; biosensor; AMPEROMETRIC BIOSENSOR; PHENOLIC-COMPOUNDS; SPECTROPHOTOMETRIC DETERMINATION; CAPILLARY-ELECTROPHORESIS; ENZYME IMMOBILIZATION; CRUDE EXTRACT; RUTIN; MICROSPHERES; QUERCETIN; ACID;
D O I：
10.1590/S0100-40422009000500021
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
IMMOBILIZATION OF LACCASE IN MICROPARTICLES OF CHITOSAN OBTAINED BY SPRAY DRYING AND USED IN THE BIOSENSORS CONSTRUCTION. Biosensors based on laccase immobilized on microparticles of chitosan crosslinked with tripolyphosphate ( biosensor I) and glyoxal ( biosensor II) obtained by spray drying for the determinations of rutin in pharmaceutical formulations were developed. Under optimized operational conditions (pH 4.0, frequency of 30 Hz, pulse amplitude of 40 mV and scan increment of 2.0 mV) two analytical curves were obtained for both biosensors showing a detection limit of 6.2x10(-8) mol L-1 for biosensor ( I) and 2.0x10(-8) mol L-1 for biosensor ( II). The recovery of rutin from pharmaceutical sample ranged from 90.7 to 105.0% and the lifetime of these biosensors were 4 months ( at least 400 determinations).
引用
收藏
页码：1195 / 1201
页数：7
相关论文
共 48 条
  • [1] An optical biosensor based on immobilization of laccase and MBTH in stacked films for the detection of catechol
    Abdullah, Jaafar
    Ahmad, Musa
    Heng, Lee Yook
    Karuppiah, Nadarajah
    Sidek, Hamidah
    [J]. SENSORS, 2007, 7 (10) : 2238 - 2250
  • [2] [Anonymous], 1995, OFFICIAL METHODS ANA, P29
  • [3] Immobilization of lipase using hydrophilic polymers in the form of hydrogel beads
    Betigeri, SS
    Neau, SH
    [J]. BIOMATERIALS, 2002, 23 (17) : 3627 - 3636
  • [4] Electrochemical approach for discriminating and measuring predominant flavonoids and phenolic acids using differential pulse voltammetry:: towards an electrochemical index of natural antioxidants
    Blasco, AJ
    González, MC
    Escarpa, A
    [J]. ANALYTICA CHIMICA ACTA, 2004, 511 (01) : 71 - 81
  • [5] The rutin/β-cyclodextrin interactions in fully aqueous solution:: spectroscopic studies and biological assays
    Calabrò, ML
    Tommasini, S
    Donato, P
    Stancanelli, R
    Raneri, D
    Catania, S
    Costa, C
    Villari, V
    Ficarra, P
    Ficarra, R
    [J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2005, 36 (05) : 1019 - 1027
  • [6] Determination of rutin and quercetin in plants by capillary electrophoresis with electrochemical detection
    Chen, G
    Zhang, HW
    Ye, JN
    [J]. ANALYTICA CHIMICA ACTA, 2000, 423 (01) : 69 - 76
  • [7] Preparation and release of salbutamol from chitosan and chitosan co-spray dried compacts and multiparticulates
    Corrigan, DO
    Healy, AM
    Corrigan, OI
    [J]. EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, 2006, 62 (03) : 295 - 305
  • [8] Industrial and biotechnological applications of laccases: A review
    Couto, Susana Rodriguez
    Herrera, Jose Luis Toca
    [J]. BIOTECHNOLOGY ADVANCES, 2006, 24 (05) : 500 - 513
  • [9] POLYMERIC MICROSPHERES AS DRUG CARRIERS
    DAVIS, SS
    ILLUM, L
    [J]. BIOMATERIALS, 1988, 9 (01) : 111 - 115
  • [10] Biosensor based on chitosan biopolymer and crude extract of ginger (Zingiber officinales rosc.) for the determination of hydroquinone in wastewater of photographic process
    de Oliveira, IRWZ
    Vieira, IC
    Lupetti, KO
    Fatibello-Filho, O
    de Fávere, VT
    Laranjeira, MCM
    [J]. ANALYTICAL LETTERS, 2004, 37 (15) : 3111 - 3127
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