Development of a transformation protocol for Tecomella undulata (Smith) Seem from cotyledonary node explants

被引：11
作者：
Aslam, Mohammad [1 ]
Singh, Rajio [1 ]
Anandhan, Sivalingam [1 ]
Pande, Veena [1 ]
Ahmed, Zakwan [1 ]
机构：
[1] Def Inst Bioenergy Res, Naini Tal 263139, Uttarakhand, India
来源：
SCIENTIA HORTICULTURAE | 2009年 / 121卷 / 01期
关键词：
Agrobacterium tumefaciens; Tecomella undulata; Cotyledonary node; MULTIPLICATION; ADAPTATION; DESERT; TREE;
D O I：
10.1016/j.scienta.2009.01.007
中图分类号：
S6 [园艺];
学科分类号：
0902 ;
摘要：
A protocol was optimized for genetic transformation of Rohida (Tecomella undulata) from cotyledonary node tissues using Agrobacterium tumefaciens strain GV2260 harboring binary vector pBinAR containing osmotin and nptII gene under control of CaMV35S promoter. This is the first report on the transformation of T. undulata. The effective concentration of selectable marker antibiotic Used for Screening of transformants was 85.97 mu M in shooting media and 42.98 mu M in rooting media. PCR and Southern blot confirmed integration of osmotin gene into genome of Rohida. (C) 2009 Elsevier B.V. All rights reserved.
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页码：119 / 121
页数：3
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