Development of a transformation protocol for Tecomella undulata (Smith) Seem from cotyledonary node explants

被引：11

作者：

Aslam, Mohammad ^{[1
]}

Singh, Rajio ^{[1
]}

Anandhan, Sivalingam ^{[1
]}

Pande, Veena ^{[1
]}

Ahmed, Zakwan ^{[1
]}

机构：

[1] Def Inst Bioenergy Res, Naini Tal 263139, Uttarakhand, India

来源：

SCIENTIA HORTICULTURAE | 2009年 / 121卷 / 01期

关键词：

Agrobacterium tumefaciens; Tecomella undulata; Cotyledonary node; MULTIPLICATION; ADAPTATION; DESERT; TREE;

D O I：

10.1016/j.scienta.2009.01.007

中图分类号：

S6 [园艺];

学科分类号：

0902 ;

摘要：

A protocol was optimized for genetic transformation of Rohida (Tecomella undulata) from cotyledonary node tissues using Agrobacterium tumefaciens strain GV2260 harboring binary vector pBinAR containing osmotin and nptII gene under control of CaMV35S promoter. This is the first report on the transformation of T. undulata. The effective concentration of selectable marker antibiotic Used for Screening of transformants was 85.97 mu M in shooting media and 42.98 mu M in rooting media. PCR and Southern blot confirmed integration of osmotin gene into genome of Rohida. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：119 / 121

页数：3

共 16 条

[1]

[Anonymous], 1989, Molecular Cloning: A Laboratory Manual

[2] CLONAL MULTIPLICATION OF TREE SPECIES IN THE THAR DESERT THROUGH TISSUE-CULTURE [J].

ARYA, HC ;

SHEKHAWAT, NS .

FOREST ECOLOGY AND MANAGEMENT, 1986, 16 (1-4) :201-208

[3]

Arya S., 1992, International Tree Crops Journal, V7, P141

[4]

Bhansali R., 1993, TROP SCI, V3, P31

[5]

Doyle J.J., 1987, Focus, V19, P11, DOI DOI 10.2307/2419362

[6] A REVISED MEDIUM FOR RAPID GROWTH AND BIO ASSAYS WITH TOBACCO TISSUE CULTURES [J].

MURASHIGE, T ;

SKOOG, F .

PHYSIOLOGIA PLANTARUM, 1962, 15 (03) :473-497

[7]

NAGAVENI HC, 2002, SEEM J TIMBER DEV AS, V48, P32

[8]

Nandwani D, 1996, GARTENBAUWISSENSCHAF, V61, P147

[9]

NANDWANI D, 1995, GARTENBAUWISSENSCHAF, V60, P65

[10] CLONAL PROPAGATION OF DESERT TEAK (TECOMELLA-UNDULATA) THROUGH TISSUE-CULTURE [J].

RATHORE, TS ;

SINGH, RP ;

SHEKHAWAT, NS .

PLANT SCIENCE, 1991, 79 (02) :217-222

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