An externally tunable bacterial band-pass filter

被引:102
作者
Sohka, Takayuki [1 ]
Heins, Richard A. [1 ]
Phelan, Ryan M. [2 ]
Greisler, Jennifer M. [1 ]
Townsend, Craig A. [2 ]
Ostermeier, Marc [1 ]
机构
[1] Johns Hopkins Univ, Dept Chem & Biomol Engn, Baltimore, MD 21212 USA
[2] Johns Hopkins Univ, Dept Chem, Baltimore, MD 21212 USA
基金
美国国家卫生研究院;
关键词
biological circuit; biological engineering; pattern formation; protein switch; synthetic biology; BETA-LACTAMASE INDUCTION; PROTEIN SWITCHES; OSCILLATOR; PATTERN; SYSTEM; GENES;
D O I
10.1073/pnas.0901246106
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The current paradigm for tuning synthetic biological systems is through re-engineering system components. Biological systems designed with the inherent ability to be tuned by external stimuli will be more versatile. We engineered Escherichia coli cells to behave as an externally tunable band-pass filter for enzyme activity and small molecules. The band's location can be positioned within a range of 4 orders of magnitude simply by the addition of compounds to the growth medium. Inclusion in the genetic network of an enzyme-substrate pair that functions as an attenuator is a generalizable strategy that enables this tunability. The genetic circuit enabled bacteria growth to be patterned in response to chemical gradients in nonintuitive ways and facilitated the isolation of engineered allosteric enzymes. The application of this strategy to other biological systems will increase their utility for biotechnological applications and their usefulness as a tool for gaining insight into nature's underlying design principles.
引用
收藏
页码:10135 / 10140
页数:6
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