Strategies for In Vivo Genome Editing in Nondividing Cells

被引：56

作者：

Nami, Fatemeharefeh ^{[1
]}

Basiri, Mohsen ^{[2
]}

Satarian, Leila ^{[2
]}

Curtiss, Cameron ^{[1
]}

Baharvand, Hossein ^{[2
,3
]}

Verfaillie, Catherine ^{[1
]}

机构：

[1] Katholieke Univ Leuven, Stamcelinst, Dept Dev & Regenerat, Leuven, Belgium

[2] Royan Inst Stem Cell Biol & Technol, ACECR, Cell Sci Res Ctr, Dept Stem Cells & Dev Biol, Tehran, Iran

[3] Univ Sci & Culture, Dept Dev Biol, Tehran, Iran

来源：

TRENDS IN BIOTECHNOLOGY | 2018年 / 36卷 / 08期

关键词：

STRAND BREAK REPAIR; RNA-GUIDED CAS9; HOMOLOGOUS RECOMBINATION; TARGETED INTEGRATION; MOUSE MODEL; STEM-CELLS; DNA; BASE; SPECIFICITY; CRISPR-CAS9;

D O I：

10.1016/j.tibtech.2018.03.004

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Programmable nucleases, including zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9), have enhanced our ability to edit genomes by the sequence-specific generation of double-strand breaks (DSBs) with subsequent homology-directed repair (HDR) of the DSB. However, the efficiency of the HDR pathway is limited in nondividing cells, which encompass most of the cells in the body. Therefore, the HDR-mediated genome-editing approach has limited in vivo applicability. Here, we discuss a mutation type-oriented viewpoint of strategies devised over the past few years to circumvent this problem, along with their possible applications and limitations.

引用

页码：770 / 786

页数：17

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