Pattern recognition analysis for 1H NMR spectra of plasma from hemodialysis patients

被引：11

作者：

Fujiwara, Masako ^{[1
,3
]}

Kobayashi, Takeshi ^{[1
]}

Jomori, Takahiro ^{[2
]}

Maruyama, Yutaka ^{[3
]}

Oka, Yoshitomo ^{[4
]}

Sekino, Hiroshi ^{[5
]}

Imai, Yutaka ^{[1
,3
]}

Takeuchi, Kazuhisa ^{[1
,5
]}

机构：

[1] Tohoku Univ, Grad Sch Pharmaceut Sci, Aoba Ku, Sendai, Miyagi 9808578, Japan

[2] Tohoku Univ, Fac Pharmaceut Sci, Sendai, Miyagi 9808578, Japan

[3] Tohoku Univ, 21st Century COE Program CRESCENDO, Sendai, Miyagi 9808578, Japan

[4] Tohoku Univ, Grad Sch Med, Div Mol Metab & Diabet, Sendai, Miyagi 9808578, Japan

[5] CKD Ctr, Koujinkai Cent Hemodialysis Clin, Sendai, Miyagi, Japan

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2009年 / 394卷 / 06期

关键词：

Lactate; Acetate; TMAO; PCA; Renal failure; Metabolomics; PROTON MAGNETIC-RESONANCE; METABOLIC CHARACTERIZATION; RENAL-FAILURE; SPECTROSCOPY; URINE; TRIMETHYLAMINE; MODEL; CLASSIFICATION; DAMAGE;

D O I：

10.1007/s00216-009-2830-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

H-1 NMR spectroscopic and pattern recognition-based methods (NMR-PR) were applied to the metabolic profiling studies on hemodialysis (HD). Plasma samples were collected from 37 patients before and after HD and measured by 600 MHz NMR spectroscopy. Each spectrum was data-processed and subjected to principal component analysis for pattern recognition. Spectral patterns of plasma between pre-and post-dialyses were clearly discriminated, together with significant fluctuations in the levels of creatinine, trimethylamine-N-oxide, glucose, lactate, and acetate, which were quantitated. We have first observed the significant elevation of lactate levels in post-dialysis plasma. The present study has demonstrated the high feasibility of NMR-PR method for monitoring the dialysis condition and comprehensive profiling of the change of low-molecular-weight metabolites in HD.

引用

页码：1655 / 1660

页数：6

共 27 条

[1] Accumulation of trimethylamine and trimethylamine-N-oxide in end-stage renal disease patients undergoing haemodialysis [J].