Fluorescence detection of natural RNA using rationally designed "clickable" oligonucleotide probes

被引：7

作者：

Okholm, Anders ^{[1
]}

Kjems, Jorgen ^{[1
]}

Astakhova, Kira ^{[2
]}

机构：

[1] Aarhus Univ, Interdisciplinary Nanosc Ctr iNANO, DK-8000 Aarhus, Denmark

[2] Univ Southern Denmark, Nucle Acid Ctr, Dept Phys Chem & Pharm, DK-5230 Odense, Denmark

来源：

RSC ADVANCES | 2014年 / 4卷 / 86期

关键词：

CIRCULAR RNAS; CYANINE DYES; DNA;

D O I：

10.1039/c4ra07165d

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Herein a reliable approach to the design of effective fluorescent probes for RNA detection is described. The fluorescence signalling of hybridization by internally positioned polyaromatic hydrocarbons and rhodamine dyes was achieved with a low fluorescence background signal, high fluorescence quantum yields at ambient and elevated temperature, high selectivity and signal specificity of the probes when binding to miR-7 and circRNA targets.

引用

页码：45653 / 45656

页数：4

共 17 条

[1] Scaffolding along Nucleic Acid Duplexes Using 2′-Amino-Locked Nucleic Acids [J].

Astakhova, I. Kira ;

Wengel, Jesper .

ACCOUNTS OF CHEMICAL RESEARCH, 2014, 47 (06) :1768-1777

[2] Interfacing Click Chemistry with Automated Oligonucleotide Synthesis for the Preparation of Fluorescent DNA Probes Containing Internal Xanthene and Cyanine Dyes [J].

Astakhova, I. Kira ;

Wengel, Jesper .

CHEMISTRY-A EUROPEAN JOURNAL, 2013, 19 (03) :1112-1122

[3] Branched DNA nanostructures efficiently stabilised and monitored by novel pyrene-perylene 2′-α-L-amino-LNA FRET pairs [J].

Astakhova, I. Kira ;

Kumar, T. Santhosh ;

Campbell, Meghan A. ;

Ustinov, Alexey V. ;

Korshun, Vladimir A. ;

Wengel, Jesper .

CHEMICAL COMMUNICATIONS, 2013, 49 (05) :511-513

[4] Tumor suppressor microRNAs: A novel non-coding alliance against cancer [J].

Blandino, Giovanni ;

Fazi, Francesco ;

Donzelli, Sara ;

Kedmi, Merav ;

Sas-Chen, Aldema ;

Muti, Paola ;

Strano, Sabrina ;

Yarden, Yosef .

FEBS LETTERS, 2014, 588 (16) :2639-2652

[5] MicroRNA functions [J].

Bushati, Natascha ;

Cohen, Stephen M. .

ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 2007, 23 :175-205

[6] Characterization of chiral H and J aggregates of cyanine dyes formed by DNA templating using stark and fluorescence spectroscopies [J].

Chowdhury, A ;

Wachsmann-Hogiu, S ;

Bangal, PR ;

Raheem, I ;

Peteanu, LA .

JOURNAL OF PHYSICAL CHEMISTRY B, 2001, 105 (48) :12196-12201

[7] Click Nucleic Acid Ligation: Applications in Biology and Nanotechnology [J].

El-Sagheer, Afaf H. ;

Brown, Tom .

ACCOUNTS OF CHEMICAL RESEARCH, 2012, 45 (08) :1258-1267

[8] Natural RNA circles function as efficient microRNA sponges [J].

Hansen, Thomas B. ;

Jensen, Trine I. ;

Clausen, Bettina H. ;

Bramsen, Jesper B. ;

Finsen, Bente ;

Damgaard, Christian K. ;

Kjems, Jorgen .

NATURE, 2013, 495 (7441) :384-388

[9] Circular RNAs: splicing's enigma variations [J].

Hentze, Matthias W. ;

Preiss, Thomas .

EMBO JOURNAL, 2013, 32 (07) :923-925

[10] Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation [J].

Hong, Vu ;

Presolski, Stanislav I. ;

Ma, Celia ;

Finn, M. G. .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2009, 48 (52) :9879-9883

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