Fluorescence detection of natural RNA using rationally designed "clickable" oligonucleotide probes

被引：7

作者：

Okholm, Anders ^{[1
]}

Kjems, Jorgen ^{[1
]}

Astakhova, Kira ^{[2
]}

机构：

[1] Aarhus Univ, Interdisciplinary Nanosc Ctr iNANO, DK-8000 Aarhus, Denmark

[2] Univ Southern Denmark, Nucle Acid Ctr, Dept Phys Chem & Pharm, DK-5230 Odense, Denmark

来源：

RSC ADVANCES | 2014年 / 4卷 / 86期

关键词：

CIRCULAR RNAS; CYANINE DYES; DNA;

D O I：

10.1039/c4ra07165d

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Herein a reliable approach to the design of effective fluorescent probes for RNA detection is described. The fluorescence signalling of hybridization by internally positioned polyaromatic hydrocarbons and rhodamine dyes was achieved with a low fluorescence background signal, high fluorescence quantum yields at ambient and elevated temperature, high selectivity and signal specificity of the probes when binding to miR-7 and circRNA targets.

引用

页码：45653 / 45656

页数：4

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