Layer-by-layer inorganic/polymeric nanoparticles for kinetically controlled multigene delivery

被引：21

作者：

Bishop, Corey J. ^{[1
]}

Liu, Allen L. ^{[1
]}

Lee, David S. ^{[1
]}

Murdock, Richard J. ^{[1
]}

Green, Jordan J. ^{[1
,2
,3
,4
,5
]}

机构：

[1] Johns Hopkins Univ, Dept Biomed Engn, Sch Med, Translat Tissue Engn Ctr, Baltimore, MD 21231 USA

[2] Johns Hopkins Univ, Dept Ophthalmol, Sch Med, Baltimore, MD 21231 USA

[3] Johns Hopkins Univ, Dept Oncol, Sch Med, Baltimore, MD 21231 USA

[4] Johns Hopkins Univ, Dept Neurosurg, Sch Med, Baltimore, MD 21231 USA

[5] Johns Hopkins Univ, Dept Mat Sci & Engn, Baltimore, MD 21231 USA

来源：

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A | 2016年 / 104卷 / 03期

基金：

美国国家卫生研究院;

关键词：

gold nanoparticle; layer-by-layer; gene delivery; poly(beta-amino ester); cell engineering; GOLD NANOPARTICLES; GENE DELIVERY; CANCER-CELLS; GROWTH; DNA; POLYPLEXES; BINDING;

D O I：

10.1002/jbm.a.35610

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Nonviral gene delivery methods represent a potential safe and effective approach for treating myriad diseases. For many gene therapy applications, delivering multiple exogenous genes and controlling the time profile that these genes are expressed would be advantageous. Polymeric nonviral gene carriers are versatile and can be readily tailored for particular therapeutic applications, have the ability to carry multiple large genes within each particle, and can be more easily manufactured than viruses used for gene delivery. A layer-by-layer (LbL) theranostic-enabling nanoparticle was developed to incorporate two plasmid types which have differing expression time profiles. Temporally controlling the expression of exogenous DNA enables superior control over the microenvironment and could lead to better control over differentiation pathways and cell fate. (c) 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 707-713, 2016.

引用

页码：707 / 713

页数：7

共 25 条

[1] Secreted Biomolecules Alter the Biological Identity and Cellular Interactions of Nanoparticles [J].