Bundling of microtubules by motor and tail domains of a kinesin-like calmodulin-binding protein from Arabidopsis:: Regulation by Ca2+/calmodulin

被引:50
作者
Kao, YL
Deavours, BE
Phelps, KK
Walker, RA
Reddy, ASN [1 ]
机构
[1] Colorado State Univ, Dept Biol, Ft Collins, CO 80523 USA
[2] Colorado State Univ, Cell & Mol Biol Program, Ft Collins, CO 80523 USA
[3] Virginia Polytech Inst & State Univ, Dept Biol, Blacksburg, VA 24061 USA
关键词
D O I
10.1006/bbrc.1999.1896
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Kinesin-like calmodulin-binding protein (KCBP), a novel kinesin-like protein from plants, is unique among kinesins and kinesin-like proteins in having a calmodulin-binding domain adjacent to its motor domain. KCBP localizes to mitotic microtubule (MT) arrays including the preprophase band, the spindle apparatus, and the phragmoplast, suggesting a role for KCBP in establishing these MT arrays by bundling MTs. To determine if KCBP bundles MTs, we expressed C-terminal motor and N-terminal tail domains of KCBP, and used the purified proteins in MT bundling assays. The 1.5 C protein with the motor and calmodulin-binding domains induced MT bundling. The 1.5 C-induced bundles were dissociated in the presence of Ca2+/calmodulin. Similar results were obtained with a 1.4 C protein, which lacks much of the coiled-coil region present in 1.5 C protein and does not form dimers. The N-terminal tail of KCBP, which contains an ATP-independent MT binding site, is also capable of bundling MTs. These results, together with the KCBP localization data, suggest the involvement of KCBP in establishing mitotic MT arrays during different stages of cell division and that Ca2+/calmodulin regulates the formation of these NIT arrays. (C) 2000 Academic Press.
引用
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页码:201 / 207
页数:7
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