Plant regeneration of Agave tequilana by indirect organogenesis

被引：34

作者：

Valenzuela-Sanchez, Karla K. ^{[1
]}

Juarez-Hernandez, Raul E. ^{[1
]}

Cruz-Hernandez, Andres ^{[1
]}

Olalde-Portugal, Victor ^{[1
]}

Valverde, Maria Elena ^{[1
]}

Paredes-Lopez, Octavio ^{[1
]}

机构：

[1] Ctr Invest & Estudios Avanzados, Unidad Irapuato, Dept Biotecnol & Bioquim, IPN, Guanajuato 36500, Mexico

来源：

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT | 2006年 / 42卷 / 04期

关键词：

Agavaceae; auxins; callus culture; meristem; tissue culture;

D O I：

10.1079/IVP2006788

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Indirect organogenesis was developed in Agave tequilana. Leaf segments and meristematic tissue from the central head ('pina') were evaluated as explant sources. A minimal-sized explant with high bud-forming capacity (19.5 BFC) was obtained through a cross section of meristematic tissue from in vitro plantlets. In callus culture, the best growth response was due to naphthalene acetic-acid (NAA) presenting a contrasting response compared to 2,4-dichlorophenoxyacetic acid (2,4-D). Regeneration from meristem segments and callus was obtained using 1.1 mu M 2,4-D and 44 mu M 6-benzylaminopurine (BA). The regeneration capacity of callus was maintained for 3 mo. Shoots regenerated were rooted in a hormone-free MS1 medium and acclimatized in a greenhouse with a 100% survival.

引用

页码：336 / 340

页数：5

共 24 条

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