Feeding status and bacterial LPS-induced cytokine and neuropeptide gene expression in hypothalamus

被引:46
作者
Gayle, D [1 ]
Ilyin, SE [1 ]
Plata-Salamán, CR [1 ]
机构
[1] Univ Delaware, Sch Life & Hlth Sci, Div Mol Biol, Newark, DE 19716 USA
关键词
food deprivation; fasting; growth factor; interleukin; nervous system; opioids; stress; tumor necrosis factor;
D O I
10.1152/ajpregu.1999.277.4.R1188
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
This study determined the effects of feeding status on basal and lipopolysaccharide (LPS)-stimulated cytokine and neuropeptide gene expression in the hypothalamus. With the use of RNase protection assays, we measured mRNA levels of interleukin-1 beta (IL-1 beta), IL-1 receptor antagonist (IL-1RA), IL-1 receptor type I (IL-1RI), IL-1R accessory proteins (AcP I and II, tumor necrosis factor-alpha (TNF-alpha), transforming growth factor-beta 1 (TGF-beta 1), glycoprotein 130 (Gp 130), leptin receptor (OB-R), neuropeptide Y (NPY), preprodynorphin, and proopiomelanocortin (POMC). Analyses were done in ad libitum-fed, fasted, and fasted and refed rats treated with the intracerebroventricular administration of physiological saline or LPS. The data show that food deprivation increases the basal mRNA expression of IL-1 beta, IL-1RA, TNF-alpha, IL-1RI, and IL-1R AcP I, whereas mRNA levels of POMC showed a decrease. Five hours of refeeding returned cytokine levels to those observed in the ad libitum-fed group. LPS administration induced a robust upregulation of IL-1 beta, TNF-alpha, and IL-1RI during all three feeding conditions. Acute food deprivation did not modulate LPS-induced changes in hypothalamic cytokine mRNA profiles. These findings show that 1) cytokine modulation and 2) as an adaptive response to the stress of acute fasting acute fasting does not affect LPS-induced cytokine mRNA modulation in the hypothalamus. The data have implications to gram-negative infections associated with acute anorexia.
引用
收藏
页码:R1188 / R1195
页数:8
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