Development of monoclonal antibodies against human CYP11B1 and CYP11B2

被引：224

作者：

Gomez-Sanchez, Celso E. ^{[1
,2
]}

Qi, Xin ^{[2
]}

Velarde-Miranda, Carolina ^{[2
]}

Plonczynski, Maria W. ^{[2
]}

Parker, C. Richard ^{[3
]}

Rainey, William ^{[4
]}

Satoh, Fumitoshi ^{[5
]}

Maekawa, Takashi ^{[5
]}

Nakamura, Yasuhiro ^{[5
]}

Sasano, Hironobu

Gomez-Sanchez, Elise P. ^{[1
,2
,6
]}

机构：

[1] GV Sonny Montgomery VA Med Ctr, Endocrine Sect, Jackson, MS 39216 USA

[2] Univ Mississippi, Med Ctr, University, MS 38677 USA

[3] Univ Alabama Birmingham, Dept Obstet & Gynecol, Birmingham, AL 35294 USA

[4] Univ Michigan, Dept Mol & Integrat Physiol, Ann Arbor, MI 48109 USA

[5] Tohoku Univ, Dept Pathol, Sendai, Miyagi, Japan

[6] Univ Mississippi, Med Ctr, Dept Pharmacol & Toxicol, Jackson, MS 39216 USA

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 2014年 / 383卷 / 1-2期

关键词：

CYP11B1; CYP11B2; Adrenal cortex; Monoclonal antibodies; 17; alpha-Hydroxylase; lmmunofluorescence; PARTIAL ALDOSTERONE RESISTANCE; MESSENGER-RNA EXPRESSION; RAT ADRENAL-CORTEX; ZONA GLOMERULOSA; GENE; DIAGNOSIS; SYNTHASE; ADENOMA; BIOSYNTHESIS; HYPERPLASIA;

D O I：

10.1016/j.mce.2013.11.022

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The final enzymes in the biosynthesis of aldosterone and cortisol are by the cytochrome P450 CYP1192 and CYP11B1, respectively. The enzymes are 93% homologous at the amino acid level and specific antibodies have been difficult to generate. 2. Mice and rats were immunized with multiple peptides conjugated to various immunogenic proteins and monoclonal antibodies were generated. The only peptide sequences that generated specific antibodies were amino acids 41-52 for the CYP11B2 and amino acids 80-90 for the CYP11B1 enzyme. 3. The mouse monoclonal CYP11B2-41 was specific and sensitive for use in western blots and produced specific staining of the zona glomerulosa of normal adrenal glands. The rat monoclonal CYP11B1-80 also detected a single band by western blot and detected only the zona fasciculata. Triple immunofluorescence of the adrenal demonstrated that the CYP11B1 and the CYP11B2 did not co-localize, while as expected the CYP11B1 co-localized with the 17 alpha-hydroxylase. Published by Elsevier Ireland Ltd.

引用

页码：111 / 117

页数：7

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