Transcriptome comparative analysis revealed poly(I:C) activated RIG-I/MDA5-mediated signaling pathway in miiuy croaker

被引：42
作者：
Chu, Qing [1 ]
Gao, Yunhang [3 ]
Xu, Guoliang [1 ]
Wu, Changwen [2 ]
Xu, Tianjun [1 ]
机构：
[1] Zhejiang Ocean Univ, Coll Marine Sci, Lab Fish Biogenet & Immune Evolut, Zhoushan 316022, Peoples R China
[2] Zhejiang Ocean Univ, Natl Engn Res Ctr Marine Facil Aquaculture, Zhoushan 316022, Peoples R China
[3] Jilin Agr Univ, Coll Anim Sci & Vet Med, Changchun 130118, Peoples R China
来源：
FISH & SHELLFISH IMMUNOLOGY | 2015年 / 47卷 / 01期
基金：
中国国家自然科学基金;
关键词：
Transcriptome; Poly(I:C); MDA5; TLR3; Signaling pathway; INTERFERON; EXPRESSION; IMMUNITY; CELLS; GENE; LGP2; TOOL;
D O I：
10.1016/j.fsi.2015.08.032
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
Miiuy croker (Miichthys miiuy) as an important economical aquaculture species has challenged many more diseases caused by various pathogens recently. To better explore the immune response to virus, we have analyzed the transcriptome profiling of miiuy croaker challenged with poly(I:C) synthetic analog of virus dsRNA. We have obtained differentially expressed genes (DEGs) with up/down-relevant from comparison of the Ctrl and Poly transcriptome libraries. Through GO and KEGG enrichment analysis, immune-relevant DEGs whose expression are significantly rise or fall after challenged have been identified and classified. In order to detailedly analysis host immune response patterns for dsRNA virus, we have performed a map based on RIG-I/MDA5-mediated and TLR3-mediated signaling pathway which both induced type I IFNs response. In this pathway, both MDA5 and LGP2 are important RLRs in host surveillance against infection of dsRNA viruses and induce type I IFNs response which subsequently form a transcription factor complex ISGF3 that promote downstream genes referred to as ISGs to inhibits virus replication. (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码：168 / 174
页数：7
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