Study on the Expression Spectrum of miRNAs in Peripheral Blood from Patients and the Value of miR-548h-5p in the Diagnosis of Chronic Obstructive Pulmonary Disease

被引：2

作者：

Li, Chun-Tao ^{[1
]}

Zhang, Jian-Qing ^{[1
]}

Dai, Lu-Ming ^{[1
]}

Zhang, Jia-Qiang ^{[1
]}

Fang, Li-Zhou ^{[1
]}

Feng, Jia-Gang ^{[1
]}

机构：

[1] Kunming Med Univ, Dept Resp Crit Care Med, Affiliated Hosp 1, Kunming 650032, Yunnan, Peoples R China

来源：

NANOSCIENCE AND NANOTECHNOLOGY LETTERS | 2020年 / 12卷 / 11期

基金：

中国国家自然科学基金;

关键词：

miRNA; COPD; Gene Microarray; Molecular Biomarkers; MESSENGER-RNA; COPD; MICRORNA; CANCER;

D O I：

10.1166/nnl.2020.3246

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

To seek novel miRNAs for the diagnosis of Chronic Obstructive Pulmonary Disease (COPD), this study analyzed the differential expression of miRNA from blood samples of COPD patients and healthy smokers (n = 3) by human microarray analysis. Then, some miRNAs were chosen for qRT-PCR validation. A total of 158 miRNAs revealed by microarray analysis have been differentially expressed between the two groups, 33 miRNAs identified to be up-regulated, whereas 125 miRNAs have been down-regulated in COPD. qRT-PCR showed the miR-548h-5p was decreased in the COPD patients compared with healthy smokers (p = 0.04). The sensitivity and specificity of miR-548h-5p to the diagnosis of COPD were 84.6% and 81.8%, respectively. In conclusion, it is recommend that miR-548h-5p as an approach to the diagnosis of COPD.

引用

页码：1323 / 1328

页数：6

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