Development, application and validation of a Taqman® real-time RT-PCR assay for the detection of infectious salmon anaemia virus (ISAV) in Atlantic salmon (Salmo salar)

被引：0

作者：

Snow, M. ^{[1
]}

McKay, P. ^{[1
]}

McBeath, A. J. A. ^{[1
]}

Black, J. ^{[1
]}

Doig, F. ^{[1
]}

Kerr, R. ^{[1
]}

Cunningham, C. O. ^{[1
]}

Nylund, A. ^{[1
]}

Devold, M. ^{[1
]}

机构：

[1] FRS Marine Lab, Aberdeen, Scotland

来源：

New Diagnostic Technology: Applications in Animal Health and Biologics Controls | 2006年 / 126卷

关键词：

infectious salmon anaemia virus; ISAV; real-time PCR; qRT-PCR; Taqman;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Infectious salmon anaemia (ISA) is a disease of cultured Atlantic salmon (Salmo salar) which was successfully eradicated from Scotland following its emergence in 1998. The rapid deployment of sensitive diagnostic methods for the detection of ISA virus (ISAV) was fundamental to the swift eradication of ISA disease in Scotland and continues to be of crucial importance to surveillance of the aquaculture industry. This study reports the development, validation, application and interpretation of two independent, highly sensitive and specific semi-quantitative Tacman (R) real-time RT-PCR (qRT-PCR) methods for the detection of ISAV. Such technology offers considerable advantages over conventional RT-PCR methods in current routine use for ISAV surveillance. These include an increased sensitivity, enhanced specificity, semi-quantification using endogenous controls, a lack of subjectivity in results interpretation, speed of processing and improved contamination control.

引用

页码：133 / 145

页数：13

共 22 条

[1] BASIC LOCAL ALIGNMENT SEARCH TOOL [J].