Single-Cell RNA Sequencing in Cancer: Lessons Learned and Emerging Challenges

被引：321

作者：

Suva, Mario L. ^{[1
,2
,3
,4
]}

Tirosh, Itay ^{[5
]}

机构：

[1] Massachusetts Gen Hosp, Dept Pathol, Boston, MA 02114 USA

[2] Massachusetts Gen Hosp, Ctr Canc Res, Boston, MA 02114 USA

[3] Harvard Med Sch, Boston, MA 02114 USA

[4] Broad Inst Harvard & MIT, Cambridge, MA 02142 USA

[5] Weizmann Inst Sci, Dept Mol Cell Biol, IL-761001 Rehovot, Israel

来源：

MOLECULAR CELL | 2019年 / 75卷 / 01期

关键词：

TRANSCRIPTOMIC ANALYSIS; SPATIAL RECONSTRUCTION; SEQ;

D O I：

10.1016/j.molcel.2019.05.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bulk genomic analyses and expression profiling of clinical specimens have shaped much of our understanding of cancer in patients. However, human tumors are intricate ecosystems composed of diverse cells, including malignant, immune, and stromal subsets, whose precise characterization is masked by bulk genomic methods. Single-cell genomic techniques have emerged as powerful approaches to dissect human tumors at the resolution of individual cells, providing a compelling approach to deciphering cancer biology. Here, we discuss some of the common themes emerging from initial studies of single-cell RNA sequencing in cancer and then highlight challenges in cancer biology for which emerging single-cell genomics methods may provide a compelling approach.

引用

页码：7 / 12

页数：6

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