Aquaporin 5 Interacts with Fluoride and Possibly Protects against Caries

被引：21

作者：

Anjomshoaa, Ida ^{[1
]}

Briseno-Ruiz, Jessica ^{[1
]}

Deeley, Kathleen ^{[1
]}

Poletta, Fernardo A. ^{[2
]}

Mereb, Juan C. ^{[3
]}

Leite, Aline L. ^{[4
]}

Barreta, Priscila A. T. M. ^{[4
]}

Silva, Thelma L. ^{[4
]}

Dizak, Piper ^{[1
]}

Ruff, Timothy ^{[1
]}

Patir, Asli ^{[5
]}

Koruyucu, Mine ^{[6
]}

Abbasoglu, Zerrin ^{[7
]}

Casado, Priscila L. ^{[8
,9
]}

Brown, Andrew ^{[1
]}

Zaky, Samer H. ^{[1
]}

Bayram, Merve ^{[5
]}

Kuechler, Erika C. ^{[1
]}

Cooper, Margaret E. ^{[1
]}

Liu, Kai ^{[10
]}

Marazita, Mary L. ^{[1
]}

Tanboga, Ilknur ^{[11
]}

Granjeiro, Jose M. ^{[8
,12
]}

Seymen, Figen ^{[6
]}

Castilla, Eduardo E. ^{[2
,13
]}

Orioli, Ieda M. ^{[14
]}

Sfeir, Charles ^{[1
]}

Owyang, Hongjiao ^{[11
]}

Buzalaf, Marilia A. R. ^{[4
]}

Vieira, Alexandre R. ^{[1
]}

机构：

[1] Univ Pittsburgh, Sch Dent Med, Dept Oral Biol, Pittsburgh, PA 15260 USA

[2] CEMIC Ctr Med Educ & Clin Res, ECLAMC Latin Amer Collaborat Study Congenital Mal, Buenos Aires, DF, Argentina

[3] Hosp Area El Bolson, ECLAMC, El Bolson, RN, Argentina

[4] Univ Sao Paulo, Bauru Sch Dent, Bauru, SP, Brazil

[5] Medipol Istanbul Univ, Dept Pedodont, Istanbul, Turkey

[6] Istanbul Univ, Dept Pedodont, Istanbul, Turkey

[7] Yeditepe Univ, Dept Pediat Dent, Fac Dent, Istanbul, Turkey

[8] Univ Fed Fluminense, Sch Dent, Clin Res Unit, Niteroi, RJ, Brazil

[9] Univ Fed Fluminense, Inst Biol, Niteroi, RJ, Brazil

[10] Univ Pittsburgh, Sch Dent Med, Dept Endodont, Pittsburgh, PA USA

[11] Marmara Univ, Dept Pediat Dent, Fac Dent, Istanbul, Turkey

[12] Natl Inst Metrol INMETRO, Niteroi, RJ, Brazil

[13] Fundacao Oswaldo Cruz, Natl Inst Populat Med Genet, Dept Genet, ECLAMC,INAGEMP,CNPq, Rio De Janeiro, Brazil

[14] Univ Fed Rio de Janeiro, Natl Inst Populat Med Genet, Dept Genet, ECLAMC,INAGEMP,CNPq Inst Biol,Ctr Hlth Sci, Rio De Janeiro, Brazil

来源：

PLOS ONE | 2015年 / 10卷 / 12期

关键词：

GENOME-WIDE ASSOCIATION; DENTAL-CARIES; SALIVARY-GLANDS; EXPRESSION; POLYMORPHISM; GENES; SCAN; LOCALIZATION; EXPERIENCE; SECRETION;

D O I：

10.1371/journal.pone.0143068

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Aquaporins (AQP) are water channel proteins and the genes coding for AQP2, AQP5, and AQP6 are clustered in 12q13. Since AQP5 is expressed in serous acinar cells of salivary glands, we investigated its involvement in caries. DNA samples from 1,383 individuals from six groups were studied. Genotypes of eight single nucleotide polymorphisms covering the aquaporin locus were tested for association with caries experience. Interaction with genes involved in enamel formation was tested. The association between enamel microhardness at baseline, after creation of artificial caries lesion, and after exposure to fluoride and the genetic markers in AQP5 was tested. Finally, AQP5 expression in human whole saliva, after exposure to fluoride in a mammary gland cell line, which is known to express AQP5, and in Wistar rats was also verified. Nominal associations were found between caries experience and markers in the AQP5 locus. Since these associations suggested that AQP5 may be inhibited by levels of fluoride in the drinking water that cause fluorosis, we showed that fluoride levels above optimal levels change AQP5 expression in humans, cell lines, and rats. We have shown that AQP5 is involved in the pathogenesis of caries and likely interacts with fluoride.

引用

页数：18

共 52 条

[1]

Abbasoglu Z, 2014, CARIES RES IN PRESS

[2] Saliva - the defender of the oral cavity [J].

Amerongen, AVN ;

Veerman, ECI .

ORAL DISEASES, 2002, 8 (01) :12-22

[3]

Anjomshoaa I, 2009, EUR J DENT, V3, P297

[4] Analysis of the association between lactotransferrin (LTF) gene polymorphism and dental caries [J].

Azevedo, Luiza Foltran ;

Pecharki, Giovana Daniela ;

Brancher, Joao Armando ;

Cordeiro Junior, Carlos Alberto ;

dos Santos Medeiros, Kamilla Gabriella ;

Antunes, Alessandra Armstrong ;

Arruda, Eduardo Silva ;

Werneck, Renata Iani ;

de Azevedo, Luciana Reis ;

Mazur, Rui Fernando ;

Moyses, Samuel Jorge ;

Moyses, Simone Tetue ;

Faucz, Fabio Rueda ;

Trevilatto, Paula Cristina .

JOURNAL OF APPLIED ORAL SCIENCE, 2010, 18 (02) :166-170

[5] Early responses to adenoviral-mediated transfer of the aquaporin-1 cDNA for radiation-induced salivary hypofunction [J].

Baum, Bruce J. ;

Alevizos, Ilias ;

Zheng, Changyu ;

Cotrim, Ana P. ;

Liu, Shuying ;

McCullagh, Linda ;

Goldsmith, Corinne M. ;

Burbelo, Peter D. ;

Citrin, Deborah E. ;

Mitchell, James B. ;

Nottingham, Liesl K. ;

Rudy, Susan F. ;

Van Waes, Carter ;

Whatley, Millie A. ;

Brahim, Jaime S. ;

Chiorini, John A. ;

Danielides, Stamatina ;

Turner, R. James ;

Patronas, Nicholas J. ;

Chen, Clara C. ;

Nikolov, Nikolay P. ;

Illei, Gabor G. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2012, 109 (47) :19403-19407

[6]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[7] Selecting a maximally informative set of single-nucleotide polymorphisms for association analyses using linkage disequilibrium [J].

Carlson, CS ;

Eberle, MA ;

Rieder, MJ ;

Yi, Q ;

Kruglyak, L ;

Nickerson, DA .

AMERICAN JOURNAL OF HUMAN GENETICS, 2004, 74 (01) :106-120

[8] A mouse caries model and evaluation of AQP5-/- knockout mice [J].

Culp, DJ ;

Quivey, RG ;

Bowen, WH ;

Fallon, MA ;

Pearson, SK ;

Faustoferri, R .

CARIES RESEARCH, 2005, 39 (06) :448-454

[9] Possible association of amelogenin to high caries experience in a guatemalan-mayan population [J].

Deeley, K. ;

Letra, A. ;

Rose, E. K. ;

Brandon, C. A. ;

Resick, J. M. ;

Marazita, M. L. ;

Vieira, A. R. .

CARIES RESEARCH, 2008, 42 (01) :8-13

[10] Expression of aquaporin isoforms during human and mouse tooth development [J].

Felszeghy, S ;

Módis, L ;

Németh, P ;

Nagy, G ;

Zelles, T ;

Agre, P ;

Laurikkala, J ;

Fejerskov, O ;

Thesleff, I ;

Nielsen, S .

ARCHIVES OF ORAL BIOLOGY, 2004, 49 (04) :247-257

← 1 2 3 4 5 6 →