Measuring the decay resistance of Scots pine heartwood indirectly by the Folin-Ciocalteu assay

To exploit the variation in the natural durability of heartwood timber, screening of a large number of trees or timber is, necessary. We suggest that the concentration of total phenolics, measured by the Folin-Ciocalteu (FC) assay, can be used to supplement or even replace in vitro decay tests for screening the variation in the resistance of Scots pine (Pinus sylvestris L.) heartwood timber against the brown-rot fungus Coniophora puteana (Schum. ex Fr.). We screened the juvenile heartwood of 520 Scots pine trees with the FC assay. Samples from 40 trees, with total phenolics concentrations ranging from 1.9 to 21.7 mg tannic acid equivalents (TAE)/g of heartwood, were subjected to an in vitro decay test. The correlation (r) between the total phenolics. concentration and mass loss was -0.82 (p < 0.001) in the 7 week long decay test. The relationship was expressed with a linear regression equation: mass loss (mg/g) = 487 - 25 x concentration of total phenolics (mg TAE/g). This strong relationship could be exploited by replacing, the decay test with the FC assay to more rapidly assess the durability of wood products.