Production of interferon-γ by microglia

被引：121

作者：

Kawanokuchi, J. ^{[1
]}

Mizuno, T. ^{[1
]}

Takeuchi, H. ^{[1
]}

Kato, H. ^{[1
]}

Wang, J. ^{[1
]}

Mitsuma, N. ^{[1
]}

Suzumura, A. ^{[1
]}

机构：

[1] Nagoya Univ, Environm Med Res Inst, Dept Neuroimmunol, Chikusa Ku, Nagoya, Aichi 4648601, Japan

来源：

MULTIPLE SCLEROSIS JOURNAL | 2006年 / 12卷 / 05期

关键词：

cytokine; EAE/MS; INF-gamma; MHC; microglia;

D O I：

10.1177/1352458506070763

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

Neural cells do not usually interact with immune cells because of the lack of major histocompatibility complex (MHC) antigen expression. Interferon-gamma (IFN-,gamma) enables this interaction via induction of MHC antigen expression in neural cells. Thus, IFN-gamma is a critical cytokine for the development of central nervous system (CNS) pathologies. IFN-,gamma, however, is considered to be produced exclusively by lymphoid cells. Here, we show for the first time that murine microglia produce IFN-gamma in response to IL-12 and/or IL-18, using RT-PCR detection of IFN-,gamma mRNA and Western blotting and immunohistochemical analysis for cytoplasmic expression of IFN-gamma. Stimulation of microglia with IL-12 and IL-18 resulted in MHC class 11 mRNA expression in microglia. Since IL-12 and IL-18 are produced in the CNS by glial cells, these cytokines may play a critical role in the initiation of neural-immune cell interaction and the induction of autoimmune processes in the CNS via induction of IFN-gamma and MHC antigens.

引用

页码：558 / 564

页数：7

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