Paenibacillus glycanilyticus sp nov., a novel species that degrades heteropolysaccharide produced by the cyanobacterium Nostoc commune

A novel bacterial strain, DS-1(T), was isolated that degrades heteropolysaccharide produced by the cyanobacterium Nostoc commune. The isolate was identified by a combination of phenotypic characterization, cellular fatty acid analysis, DNA base composition, DNA-DNA hybridization and 16S rRNA gene sequence analysis. Phylogenetic analysis placed strain DS-1(T) within the Paenibacillus cluster on a phylogenetic tree and the phenotypic characteristics of this strain appear to be similar to those of Paenibacillus curdlanolyticus IFO 15724(T) and Paenibacillus kobensis IFO 15729(T). The strain was distinguished from A curdlanolyticus IFO 15724(T) and A kobensis IFO 15729(T) by its ability to degrade the polysaccharide of Nostoc commune, by assimilation of rhamnose, inositol and L-fucose and by its wide range of optimal growth temperature (28-37degreesC). Like other Paenibacillus species, this strain contains anteiso-C-15:0 as a major cellular fatty acid, and it has a DNA G+C content of 50.5 mol %. Based on these results, it is concluded that this isolate should be placed within a novel species of Paenibacillus, Paenibacillus glycanilyticus sp. nov., with the type strain DS-1(T)(=IFO 16618(T)=JCM 11221(T)=NRRL B-23455(T)).