Integrin alpha5beta1 suppresses rBMSCs anoikis and promotes nitric oxide production

被引：28
作者：
Chen, Hai-ying [1 ,2 ]
Pan, Li [1 ,2 ]
Yang, Hong-li [1 ,2 ]
Xia, Peng [3 ,4 ]
Yu, Wan-cheng [5 ]
Tang, Wen-qiang [1 ,2 ]
Zhang, Ying-xin [1 ,2 ]
Chen, Shuang-feng [1 ,2 ]
Xue, Yu-zeng [3 ,4 ]
Wang, Le-xin [3 ,4 ,6 ]
机构：
[1] Liaocheng Peoples Hosp, Cent Lab, Liaocheng 252000, Shandong, Peoples R China
[2] Liaocheng Peoples Hosp, Key Lab Oral & Maxillofacial Head & Neck Med Biol, Liaocheng 252000, Shandong, Peoples R China
[3] Taishan Med Univ, Dept Cardiol, Liaocheng Peoples Hosp, Liaocheng 252000, Shandong, Peoples R China
[4] Taishan Med Univ, Clin Sch, Liaocheng 252000, Shandong, Peoples R China
[5] Shandong Univ, Prov Hosp, Dept Cardiac Surg, Jinan 250000, Shandong, Peoples R China
[6] Charles Sturt Univ, Sch Biomed Sci, Wagga Wagga, NSW 2650, Australia
来源：
BIOMEDICINE & PHARMACOTHERAPY | 2018年 / 99卷
基金：
中国国家自然科学基金;
关键词：
Bone marrow derived mesenchymal stem cells; Anoikis; Integrin; Nitric oxide; Pulmonary arterial hypertension; MESENCHYMAL STEM-CELLS; PULMONARY ARTERIAL-HYPERTENSION; SYNTHASE; EXPRESSION; SURVIVAL; ADHESION; RAT;
D O I：
10.1016/j.biopha.2018.01.038
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Background: Cell based therapy has been heralded as a novel, promising therapeutic strategy for cardiovascular diseases including pulmonary arterial hypertension (PAH). However, the low survival rate after transplantation due to cell death via anoikis is a major obstacle in stem cell therapy. Cells adhesion via Integrin alpha5beta1 (ITGA5B1) has a tendency to exert higher maximum forces. The present study aimed to evaluate the potential protective effect of ITGA5B1 on rat bone marrow mesenchymal stem cells (rBMSCs) from anoikis. Methods: Mononuclear cells were isolated by density gradient centrifugation with Ficoll, and rBMSCs cell surface markers were evaluated by flow cytometry. Osteogenic and adipocyte differentiation was determined by Alizarin Red S and Oil Red O staining respectively. The expression of Integrin A5 (ITGA5), Integrin B1 (ITGB1), eNOS and actived-caspase-3 mRNA or protein was confirmed by qPCR and western-blot. Cell adhesion, cell viability, anoikis and the migration of rBMSCs were also evaluated. Nitric oxide (NO) production was detected by the greiss assay. Results: Co-infected with Integrin A5 and B1 lentivirus to rBMSCs increased ITGA5 and ITGB1 mRNA and protein expression. ITGA5B1 enhanced the cell adhesion, cell viability, cell migration and NO production but reduced the cell anoikis in rBMSCs/ITGA5B1 groups. Conclusion: Transduction of rat rBMSCs with ITGA5B1 lentivirus could prevent cell anoikis and increase NO production.
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页码：1 / 8
页数：8
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