Piezoelectric materials have many advantages, such as: high stiffness, a high frequency response, and high resolution. It has been widely used in reducing vibration system, but hysteresis confines its use. This paper aims to develop the hysteresis model for a single axis piezoelectric nanopositioner stage at different operating points. The adopted methodology is to compare between the analytical model using MATLAB and the real time results using LabVIEW. The experimental results for real measurements had been acquired for the piezoelectric nanopositioner stage using data acquisition system from National Instruments. The experimental setup is described and output results for simulation and real time measurements are compared to validate the adopted approach.