Detection of DSP-toxins, okadaic acid, and dinophysis toxin-1 in shellfish by serine/threonine protein phosphatase assay

被引：0
|
作者：
Honkanen, RE [1 ]
Mowdy, DE [1 ]
Dickey, RW [1 ]
机构：
[1] US FDA, OFF SEAFOOD, DAUPHIN ISL, AL 36528 USA
来源：
JOURNAL OF AOAC INTERNATIONAL | 1996年 / 79卷 / 06期
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D O I：
暂无
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A relatively rapid protein phosphatase-based assay was developed for detecting okadaic acid in extracts of shellfish, using oysters (Crassostrea virginica) as a model, The assay has good sensitivity detecting okadaic acid in crude methanolic extracts of oysters at greater than or equal to 4 ng/ml (greater than or equal to 0.1 ng/assay). Assay accuracy for detecting toxic shellfish was validated through a series of spike recovery experiments, In more than 320 assessments, all oysters containing toxic amounts of okadaic acid (greater than or equal to 0.2 mu g/g) were detected, Results of analysis of the same extract by phosphatase assay and liquid chromatography gave very high correlation.
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页码：1336 / 1343
页数：8
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