Abscisic acid stimulates the expression of two isoforms of plasma membrane Ca2+-ATPase in Arabidopsis thaliana seedlings

被引:39
作者
Cerana, M.
Bonza, M. C.
Harris, R.
Sanders, D.
De Michelis, M. I.
机构
[1] Univ Milan, Dipartimento Biol, CNR, Ist Biofis,Sez Milano, I-20133 Milan, Italy
[2] Univ York, Dept Biol, York YO10 5YW, N Yorkshire, England
关键词
gene expression; plant type IIBCa2+-ATPases; abscisic acid; RT-PCR; real-time PCR; plasma membrane;
D O I
10.1055/s-2006-924111
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
At-ACA8 and At-ACA9 are two plasma membrane (PM) Ca2+-ATPases of Arabidopsis thaliana. In this article the expression of At-ACA8, At-ACA9, and of At-ACA10, a third isoform of Ca2+-ATPase closely related to PM Ca2+-ATPases, was analysed and the effect of the hormone abscisic acid (ABA) on the expression level of PM Ca2+-ATPase specific transcripts was investigated. in adult plants of A. thaliana, At-ACA8 and At-ACA10 are expressed in all organs considered whereas At-ACA9 is expressed only in flowers. All isoforms of PM Ca2+-ATPases can be detected in young seedlings but the amount of At-ACA9 mRNA is much lower than those of At-ACA8 and At-ACA10. ABA markedly and rapidly stimulates the expression of both At-ACA8 and At-ACA9 genes in young seedlings but not that of At-ACA10. ABA also increases the level of At-ACA8 protein at the PM, suggesting a role for PM Ca2+-ATPases in ABA signalling.
引用
收藏
页码:572 / 578
页数:7
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