A framework to refine particle clusters produced by EMAN

被引：0
作者：
Fan, Liya [1 ,2 ]
Zhang, Fa [1 ]
Wang, Gongming [1 ,2 ]
Liu, Zhiyong [1 ]
机构：
[1] Chinese Acad Sci, Inst Comp Technol, Beijing, Peoples R China
[2] Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
来源：
BIOINFORMATICS | 2009年 / 25卷 / 12期
关键词：
SPIDER;
D O I：
10.1093/bioinformatics/btp219
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Motivation: EMAN is one of the most popular software packages for single particle reconstruction. But the particle clusters produced during its model re. ning stage are of low qualities. We attempt to re. ne the particle clusters by more accurately determining orientations of particles, and thereby achieving higher resolutions of consequent 3D structures. Results: A particle reclustering framework (PRF) is introduced, which consists of three components. Each of them is responsible for one of the basic tasks of PRF: normalization, threshold determination and reclustering. Our implementation is also described and proved to meet the constraints proposed by PRF. Experiments revealed that our implementation improved resolutions of consequent structures for most cases, but only a little extra execution time was incurred. Therefore, it is practical to incorporate PRF in EMAN to improve qualities of generated 3D structures.
引用
收藏
页码：I276 / I280
页数：5
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