Characterization of equine and other vertebrate TLR3, TLR7, and TLR8 genes

被引：30

作者：

Astakhova, Natalia M. ^{[1
]}

Perelygin, Andrey A. ^{[1
,2
]}

Zharkikh, Andrey A.

Lear, Teri L. ^{[3
]}

Coleman, Stephen J. ^{[3
]}

MacLeod, James N. ^{[3
]}

Brinton, Margo A. ^{[1
]}

机构：

[1] Georgia State Univ, Dept Biol, Atlanta, GA 30302 USA

[2] Myriad Genet Inc, Bioinformat Dept, Salt Lake City, UT 84108 USA

[3] Univ Kentucky, Dept Vet Sci, Lexington, KY 40546 USA

来源：

IMMUNOGENETICS | 2009年 / 61卷 / 07期

关键词：

Equine TLR3; Equine TLR7; Equine TLR8; Single-nucleotide polymorphisms; FISH; Phylogenetic tree; TOLL-LIKE RECEPTOR-3; SINGLE-STRANDED RNA; EXPRESSION; CONVERSION; CLONING; TOLL-LIKE-RECEPTOR-8; IDENTIFICATION; POLYMORPHISMS; RECOGNITION; MORTALITY;

D O I：

10.1007/s00251-009-0381-z

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Toll-like receptors 3, 7, and 8 (TLR3, TLR7, and TLR8) were studied in the genomes of the domestic horse and several other mammals. The messenger RNA sequences and exon/intron structures of these TLR genes were determined. An equine bacterial artificial chromosome clone containing the TLR3 gene was assigned by fluorescent in situ hybridization to the horse chromosomal location ECA27q16-q17 and this map location was confirmed using an equine radiation hybrid panel. Direct sequencing revealed 13 single-nucleotide polymorphisms in the coding regions of the equine TLR 3, 7, and 8 genes. Of these polymorphisms, 12 were not previously reported. The allelic frequency was estimated for each single-nucleotide polymorphism from genotyping data obtained for 154 animals from five horse breeds. Some of these frequencies varied significantly among different horse breeds. Domain architecture predictions for the three equine TLR protein sequences revealed several conserved regions within the variable leucine-rich repeats between the corresponding horse and cattle TLR proteins. A phylogenetic analysis did not indicate that any significant exchanges had occurred between paralogous TLR7 and TLR8 genes in 20 vertebrate species analyzed.

引用

页码：529 / 539

页数：11

共 39 条

[21] A functional toll-like receptor 8 variant is associated with HIV disease restriction [J].

Oh, D. -Y. ;

Taube, S. ;

Hamouda, O. ;

Kuecherer, C. ;

Poggensee, G. ;

Jessen, H. ;

Eckert, J. K. ;

Neumann, K. ;

Storek, A. ;

Pouliot, M. ;

Borgeat, P. ;

Oh, N. ;

Schreier, E. ;

Pruss, A. ;

Hattermann, K. ;

Schumann, R. R. .

JOURNAL OF INFECTIOUS DISEASES, 2008, 198 (05) :701-709

[22] Cloning of canine Toll-like receptor 7 gene and its expression in dog tissues [J].

Okui, Yasuhumi ;

Kano, Rui ;

Maruyama, Haruhiko ;

Hasegawa, Atsuhiko .

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 2008, 121 (1-2) :156-160

[23] Structure of equine 2′-5′oligoadenylate synthetase (OAS)gene family and FISH mapping of OAS genes to ECA8p15→p14 and BTA17q24→q25 [J].

Perelygin, AA ;

Lear, TL ;

Zharkikh, AA ;

Brinton, MA .

CYTOGENETIC AND GENOME RESEARCH, 2005, 111 (01) :51-56

[24] Comparative analysis of vertebrate EIF2AK2 (PKR) genes and assignment of the equine gene to ECA15q24-q25 and the bovine gene to BTA11q12-q15 [J].

Perelygin, Andrey A. ;

Lear, Teri L. ;

Zharkikh, Andrey A. ;

Brinton, Margo A. .

GENETICS SELECTION EVOLUTION, 2006, 38 (05) :551-563

[25] Identification and characterization of a functional, alternatively spliced Toll-like receptor 7 (TLR7) and genomic disruption of TLR8 in chickens [J].

Philbin, VJ ;

Iqbal, M ;

Boyd, Y ;

Goodchild, MJ ;

Beal, RK ;

Bumstead, N ;

Young, J ;

Smith, AL .

IMMUNOLOGY, 2005, 114 (04) :507-521

[26] A 4,103 marker integrated physical and comparative map of the horse genome [J].

Raudsepp, T. ;

Gustafson-Seabury, A. ;

Durkin, K. ;

Wagner, M. L. ;

Goh, G. ;

Seabury, C. M. ;

Brinkmeyer-Langford, C. ;

Lee, E. -J. ;

Agarwala, R. ;

Stallknecht-Rice, E. ;

Schaeffer, A. A. ;

Skow, L. C. ;

Tozaki, T. ;

Yasue, H. ;

Penedo, M. C. T. ;

Lyons, L. A. ;

Khazanehdari, K. A. ;

Binns, M. M. ;

MacLeod, J. N. ;

Distl, O. ;

Guerin, G. ;

Leeb, T. ;

Mickelson, J. R. ;

Chowdhary, B. P. .

CYTOGENETIC AND GENOME RESEARCH, 2008, 122 (01) :28-36

[27] Exceptional conservation of horse-human gene order on X chromosome revealed by high-resolution radiation hybrid mapping [J].

Raudsepp, T ;

Lee, EJ ;

Kata, SR ;

Brinkmeyer, C ;

Mickelson, JR ;

Skow, LC ;

Womack, JE ;

Chowdhary, BP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (08) :2386-2391

[28] Molecular identification and functional expression of porcine Toll-like receptor (TLR) 3 and TLR7 [J].

Sang, Yongming ;

Yang, Jun ;

Ross, Chris R. ;

Rowland, Raymond R. R. ;

Blecha, Frank .

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 2008, 125 (1-2) :162-167

[29] Genetic analysis of Toll/Interleukin-1 Receptor (TIR) domain sequences from rhesus macaque Toll-like receptors (TLRs) 1-10 reveals high homology to human TLR/TIR sequences [J].

Sanghavi, SK ;

Shankarappa, R ;

Reinhart, TA .

IMMUNOGENETICS, 2004, 56 (09) :667-674

[30] Association of TLR7 single nucleotide polymorphisms with chronic HCV-infection and response to interferon-a-based therapy [J].

Schott, E. ;

Witt, H. ;

Neumann, K. ;

Bergk, A. ;

Halangk, J. ;

Weich, V. ;

Mueller, T. ;

Puhl, G. ;

Wiedenmann, B. ;

Berg, T. .

JOURNAL OF VIRAL HEPATITIS, 2008, 15 (01) :71-78

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